eBioscience™ 7-AAD Viability Staining Solution

eBioscience™ 7-AAD Viability Staining Solution - FAQs

2 个常见问题解答

How should I store the eBioscience 7-AAD Viability Staining Solution (Cat. No. 00-6993-50)?

We recommend storing the eBioscience 7-AAD Viability Staining Solution (Cat. No. 00-6993-50) at 2-8 degrees C, protected from light.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I discriminate between live cells and dead cells when doing a flow cytometry analysis?

Discrimination between viable and non-viable cells can be carried out with the use of the 7-AAD Viability Dye (Cat. No. 00-6993-50) or Propidium Iodide (PI) Staining Solution (Cat. No. 00-6990-50). The 7-AAD or PI will mark the non-viable cells by binding to the nuclei of those cells. The nucleic acid of viable cells will not be accessible to the dye and will not be stained. When analyzing the data collected, gate out all cells stained with the viability dye. When staining for intracellular proteins, use the Fixable Viability Dye eFluor 455UV, 450, 520, 660, or 780.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.