Phosphorylation of the human estrogen receptor. Identification of hormone-regulated sites and examination of their influence on transcriptional activity.
AuthorsLe Goff P, Montano MM, Schodin DJ, Katzenellenbogen BS
JournalJ Biol Chem
PubMed ID8308015
'We have used a transient transfection system with a cytomegalovirus-based vector expressing high levels of biologically active human estrogen receptor (ER) in COS-1 cells to study the phosphorylation of human ER and to identify major hormone-regulated phosphorylation sites. The features of phosphorylation of the wild-type ER were very similar to ... More
Distribution of tight junction proteins in adult human salivary glands.
AuthorsMaria OM, Kim JW, Gerstenhaber JA, Baum BJ, Tran SD
JournalJ Histochem Cytochem
PubMed ID18765838
'Tight junctions (TJs) are an essential structure of fluid-secreting cells, such as those in salivary glands. Three major families of integral membrane proteins have been identified as components of the TJ: claudins, occludin, and junctional adhesion molecules (JAMs), plus the cytosolic protein zonula occludens (ZO). We have been working to ... More
A role for estrogen receptor beta in the regulation of growth of the ventral prostate.
AuthorsWeihua Z, Makela S, Andersson LC, Salmi S, Saji S, Webster JI, Jensen EV, Nilsson S, Warner M, Gustafsson JA,
JournalProc Natl Acad Sci U S A
PubMed ID11371645
In normal rats and mice, immunostaining with specific antibodies revealed that nuclei of most prostatic epithelial cells harbor estrogen receptor beta (ERbeta). In rat ventral prostate, 530- and 549-aa isoforms of the receptor were identified. These sediment in the 4S region of low-salt sucrose gradients, indicating that prostatic ERbeta does ... More
Bromodeoxyuridine immunohistochemistry of epon-embedded undecalcified bone in a canine fracture healing model.
AuthorsXiang Z, Markel MD
JournalJ Histochem Cytochem
PubMed ID7769234
We evaluated bromodeoxyuridine (BrdU) immunohistochemistry of paraffin-, methyl methacrylate (MMA)-, and epon-araldite (epon)-embedded canine bone specimens to establish an optimal technique for studying cell kinetics of fracture healing in a canine tibial gap model. Dogs were sacrificed 4 months after tibial ostectomy and 1 hr after i.v. injection of BrdU ... More