123计数 eBead™ 计数微球

123计数的 eBead 可用于通过流式细胞分析对细胞或其他微粒的绝对计数。这些 7 μm 微粒包含兼容蓝色 (488 nm) 和紫色了解更多信息

货号	数量
01-1234-42	100 tests

货号 01-1234-42

价格（CNY)

2,148.00

Ends: 31-Dec-2025

2,854.00

共减 706.00 (25%)

添加至购物车

100 tests

价格（CNY)

2,148.00

Ends: 31-Dec-2025

2,854.00

共减 706.00 (25%)

添加至购物车

123计数的 eBead 可用于通过流式细胞分析对细胞或其他微粒的绝对计数。这些 7 μm 微粒包含兼容蓝色 (488 nm) 和紫色 (405 nm) 激发源的封装染料，发射约 500 nm 至 750 nm 之间的荧光。它们以已知浓度溶于含 2 mM 叠氮化钠的 0.05% Tween-20 中。在流式细胞仪上仔细收集悬浮和测量数量的细胞:磁珠混合物，可实现细胞的比率式计数。

报告的应用
流式细胞分析

仅供科研使用。不可用于诊断程序。

适用于（设备）流式细胞仪

测试数量100 tests

数量100 tests

产品线eBeads™

产品类型细胞计数微珠

Unit SizeEach

常见问题解答 (FAQ)

我用计数微珠进行细胞计数，但在散点图上找不到微珠，该怎么办？

首先检查您的阈值，看它是否设置为前向散射。如果是的话，微珠可能被阈值排除。降低阙值设置后，应该就可以显示出您的微珠了。

我想用流式细胞仪计数细胞，我该怎么做？

可以向样品中添加内部微球计数的标准品来完成流式细胞仪细胞计数。收集到的微球数量已知体积。这使得您可以计算细胞浓度。

I am using counting beads to count cells, but I cannot find the beads on my scatter plots. What do I do?

The first thing to do is check your threshold and see if it is set on forward scatter. If so, the beads are probably being excluded by the threshold. Reducing the threshold setting should reveal your beads.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to count my cells using flow cytometry. How do I do this?

Cell counting using flow cytometry can be accomplished by adding an internal microsphere counting standard to the flow cytometric sample. The number of reference beads that are collected reflects a known volume. This allows you to calculate cell concentration.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What does yield and purity mean in reference to the MagniSort kits? How are each of these parameters calculated?

Yield is the percentage of target cells harvested based on the number of cells theoretically available in the starting population. To calculate yield, stain a sample of unsorted and sorted cells with antibodies to identify the target cells, and count the total number of target cells using 123count eBeads (Cat. No. 01-1234-42) on the cytometer. Yield = # of sorted target cells, # of target cells unsorted.

Purity is the percentage of cells harvested that are target cells. Purity can be directly generated by flow cytometric analysis of sorted samples. To obtain purity, stain the sorted cells with antibodies to identify the target cells and analyze by flow cytometry. Gate on live, single cells, and subsequently gate on the target cell population. Purity is the percentage of target cells in the live, single-cell gate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.