Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin.
AuthorsCoene ED, Shaw MK, Vaux DJ,
JournalMethods Mol Biol
PubMed ID18287658
A number of endogenously biotinylated proteins are found in both cytosol and mitochondria of mammalian cells from many tissues, including liver, spleen, pancreas, kidney, and intestine. Therefore, caution should be taken when using the biotin detection system. Endogenous biotin can interfere with staining systems that employ the use of biotin-avidin- ... More
Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques.
AuthorsMcKay BE, Molineux ML, Turner RW,
JournalMethods Mol Biol
PubMed ID18287654
The interaction between avidin and biotin or streptavidin and biotin forms the basis of several widely used immunohistochemical techniques. An assumption inherent to these techniques is that endogenous biotin is not present in the tissue in detectable quantities, as neither avidin nor streptavidin can discriminate between endogenous biotin and biotinylated ... More