Live attenuated lentivirus infection elicits polyfunctional simian immunodeficiency virus Gag-specific CD8+ T cells with reduced apoptotic susceptibility in rhesus macaques that control virus replication after challenge with pathogenic SIVmac239.
AuthorsGenescà M, Rourke T, Li J, Bost K, Chohan B, McChesney MB, Miller CJ,
JournalJ Immunol
PubMed ID17878372
'HIV-specific CD8+ T cells that secrete multiple cytokines in response to Ag stimulation are associated with the control of virus replication during chronic HIV infection. To determine whether the presence of polyfunctional CD8+ T cell responses distinguishes protected and unprotected monkeys in a live attenuated lentivirus model, SIV Gag peptide-specific ... More
Performance of serum-supplemented and serum-free media in IFNgamma Elispot Assays for human T cells.
AuthorsJanetzki S, Price L, Britten CM, van der Burg SH, Caterini J, Currier JR, Ferrari G, Gouttefangeas C, Hayes P, Kaempgen E, Lennerz V, Nihlmark K, Souza V, Hoos A,
JournalCancer Immunol Immunother
PubMed ID19894047
'The choice of serum for supplementation of media for T cell assays and in particular, Elispot has been a major challenge for assay performance, standardization, optimization, and reproducibility. The Assay Working Group of the Cancer Vaccine Consortium (CVC-CRI) has recently identified the choice of serum to be the leading cause ... More
[Study of adoptive immunotherapy for metastatic renal cell carcinoma with lymphokine-activated killer (LAK) cells and interleukin-2. II. Clinical evaluation]
AuthorsNomura K, Fujioka T,
JournalNippon Hinyokika Gakkai Zasshi
PubMed ID8320888
'We evaluated adoptive immunotherapy using LAK cells combined with systemic administration of interleukin-2 (IL-2) in 11 patients with metastatic renal cell carcinoma. The LAK cells were generated by incubation in serum-free medium (AIM-V) supplemented with IL-2 (1,000 U/ml) for 4 days and were generally administered twice weekly (4 times/cycle). Daily ... More
Abnormal acidification of melanoma cells induces tyrosinase retention in the early secretory pathway.
Authors Halaban Ruth; Patton Robin S; Cheng Elaine; Svedine Sherri; Trombetta E Sergio; Wahl Miriam L; Ariyan Stephen; Hebert Daniel N;
JournalJ Biol Chem
PubMed ID11812790
'In tyrosinase-positive amelanotic melanoma cells, inactive tyrosinase accumulates in the endoplasmic reticulum. Based on studies described here, we propose that aberrant vacuolar proton ATPase (V-ATPase)-mediated proton transport in melanoma cells disrupts tyrosinase trafficking through the secretory pathway. Amelanotic but not melanotic melanoma cells or normal melanocytes display elevated proton export ... More
Serum-free culture medium and IL-7 costimulation increase the sensitivity of ELISpot detection.
AuthorsMartinuzzi E, Scotto M, Enée E, Brezar V, Ribeil JA, van Endert P, Mallone R
JournalJ Immunol Methods
PubMed ID18242633
'The identification of parameters maximizing detection sensitivity in ELISpot assays is important to transfer this technology into the clinical setting for identifying rare Ag-specific CD8(+) T cells. We have therefore considered human IFN-gamma CD8(+) T cell responses against viral epitopes to analyze different variables which could be critical during the ... More
CD4 T cells guarantee optimal competitive fitness of CD8 memory T cells.
AuthorsJohansen P, Stamou P, Tascon RE, Lowrie DB, Stockinger B,
JournalEur J Immunol
PubMed ID14971034
We studied the contribution of CD4 T cell help to survival and competitive fitness of CD8 memory T cells specific for influenza virus nucleoprotein. In agreement with recent studies, the optimal generation of functional memory CD8 T cells required CD4 help, although long-term maintenance of resting CD8 memory T cells ... More
CCL2 increases X4-tropic HIV-1 entry into resting CD4+ T cells.
AuthorsCampbell GR, Spector SA,
JournalJ Biol Chem
PubMed ID18784079
During human immunodeficiency virus type 1 (HIV-1) infection, there is a strong positive correlation between CCL2 levels and HIV viral load. To determine whether CCL2 alters HIV-1 infection of resting CD4(+) T cells, we infected purified resting CD4(+) T cells after incubation with CCL2. We show that CCL2 up-regulates CXCR4 ... More
A serum-free medium for human primary T lymphocyte culture.
AuthorsCausey AL, Wooten RM, Clem LW, Bly JE,
JournalJ Immunol Methods
PubMed ID7930634
This paper describes a defined serum-free medium (SFM), designated A-X, which supports in vitro proliferation of human primary T cells stimulated with mitogens, two-way mixed leukocyte reactions, anti-CD3, and a superantigen. A-X is a 1:1 mixture of two commercially available SFM, AIM V and EX-CELL 300. In each assay tested ... More
Retrovirus-mediated gene transfer into CD4+ and CD8+ human T cell subsets derived from tumor-infiltrating lymphocytes and peripheral blood mononuclear cells.
AuthorsMorecki S, Karson E, Cornetta K, Kasid A, Aebersold P, Blaese RM, Anderson WF, Rosenberg SA,
JournalCancer Immunol Immunother
PubMed ID2007247
Studies were undertaken to test the susceptibility of individual T cell subpopulations to retroviral-mediated gene transduction. Gene transfer into human tumor-infiltrating lymphocytes (TIL) or peripheral blood mononuclear cells (PBMC) was carried out by transduction with an amphotropic murine retroviral vector (LNL6 or N2) containing the bacterial neoR gene. The presence ... More
[Effect of different media on long-term cultivation of human synovial macrophages]
AuthorsBurg S, Zschäbitz A, Stofft E,
JournalZ Rheumatol
PubMed ID1718099
Cells of rheumatoid synovial tissue were bred in dispersion culture. In order to investigate the influence of three different culture media on the maturation and vitality of synovio-macrophages, Ham F10 (+fetal calf serum) and RPMI 1640 (+pooled AB Rh+ human serum) were compared with the serum- and cytokin-free solution AIM ... More
Human T-cell responses to Trichophyton tonsurans: inhibition using the serum free medium Aim V.
AuthorsSlunt JB, Taketomi EA, Platts-Mills TA,
JournalClin Exp Allergy
PubMed ID9383259
Proteins of the fungus Trichophyton tonsurans have been shown to give strong T cell proliferative responses in vitro using lymphocytes from individuals with immediate or delayed skin tests. Furthermore, Trichophyton-specific T-cell lines produce distinct patterns of cytokine production depending upon the skin-test reactivity of the host. However, skin-test negative individuals ... More
Development of a clinical model for ex vivo expansion of multiple populations of effector cells for adoptive cellular therapy.
BACKGROUND: We have previously demonstrated a laboratory model for expanding autologous mononuclear cells into populations of effector killer cells. The goal of the current experiments was to develop a good manufacturing practice (GMP) method for expanding clinical-grade activated effector cells that mediate tumor cell killing through various mechanisms that could ... More
Increased frequency of CD3/8/56-positive umbilical cord blood T lymphocytes after allo-priming in vitro.
Umbilical cord blood (UCB) or adult peripheral blood mononuclear cells (PBMC) were repeatedly stimulated by HLA-mismatched allogeneic Epstein-Barr virus (EBV)-transformed cell lines, and the bulk responders or single-cloned cells were immunophenotypically analyzed by flow cytometry. One month after the allo-stimulation, not in PBMC but in UCB, the proportion of CD3/8/56 ... More
A comparison of serum-free media for the support of in vitro mitogen-induced blastogenic expansion of cytolytic lymphocytes.
AuthorsMcVicar DW, Li F, McCrady CW, Merchant RE,
JournalCytotechnology
PubMed ID1367404
The high cost and potential dangers of including human AB serum in incubation media used to expand lymphocyte populations in vitro for adoptive immunotherapy have stimulated efforts to develop defined media which can support both the expansion and induction of lymphocytes with tumor cytolytic activity in the absence of serum. ... More
Simplified process for the production of anti-CD19-CAR-engineered T cells.
AuthorsTumaini B, Lee DW, Lin T, Castiello L, Stroncek DF, Mackall C, Wayne A, Sabatino M,
Journal
PubMed ID23992830
Adoptive immunotherapy with the use of chimeric antigen receptor (CAR)-engineered T cells specific for CD19 has shown promising results for the treatment of B-cell lymphomas and leukemia. This therapy involves the transduction of autologous T cells with a viral vector and the subsequent cell expansion. We describe a new, simplified ... More
Induction of primary anti-HIV CD4 and CD8 T cell responses by dendritic cells transduced with self-inactivating lentiviral vectors.
AuthorsChen X, Wang B, Chang LJ,
JournalCell Immunol
PubMed ID17188256
In this study, we demonstrate that a minimal self-inactivating (SIN) lentiviral vector (LV) that does not encode any human immunodeficiency virus (HIV) genes is able to induce HIV-specific CD4 and CD8 T cell responses after transduction of dendritic cells (DCs). The LV-DC-primed T cells displayed HIV-specific lytic degranulation, as illustrated ... More
An improved procedure for the development of human mast cells from dispersed fetal liver cells in serum-free culture medium.
Authors Kambe N; Kambe M; Chang H W; Matsui A; Min H K; Hussein M; Oskerizian C A; Kochan J; Irani A A; Schwartz L B;
JournalJ Immunol Methods
PubMed ID10854605
The in vitro development of human mast cells from fetal liver cells with recombinant human stem cell factor in serum-containing RPMI was compared to that in AIM-V media with and without serum. Compared to serum-containing media, AIM-V medium caused mast cells to develop earlier and in greater numbers. By 2 ... More
Enhancement of lymphocyte proliferation assays by use of serum-free medium.
Authors Kaldjian E P; Chen G H; Cease K B;
JournalJ Immunol Methods
PubMed ID1532181
Fetal bovine serum (FBS)-supplemented cell culture medium has been the standard medium used in assays of murine lymphocyte proliferation. While FBS allows cell growth and viability, it requires screening and contains uncharacterized elements which may yield inconsistent results from batch to batch. It also may include growth factors and immunologically ... More
Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V.
AuthorsFujita H, Shimizu K, Nagamori E
JournalBiotechnol Bioeng
PubMed ID19350625
We have fabricated muscle tissue from murine myoblast cell line C2C12 by modifying the previously reported method. Fabrication of skeletal muscle tissue has been performed in many ways including the use of a biodegradable scaffold, a collagen gel-embedded culture, or cell sheet tissue engineering, but the extent of tension generation ... More
Large-scale expansion in interleukin-2 of tumor-infiltrating lymphocytes from patients with ovarian carcinoma for adoptive immunotherapy.
Authors Freedman R S; Tomasovic B; Templin S; Atkinson E N; Kudelka A; Edwards C L; Platsoucas C D;
JournalJ Immunol Methods
PubMed ID8308273
Tumor infiltrating lymphocytes (TIL) from malignant ascites or solid tumor specimens obtained from patients with ovarian carcinoma were expanded to large numbers in vitro (10(10)-10(11)) by a four-step method using AIM V medium and low concentrations of recombinant interleukin-2 (rIL-2). The expansion procedure employed 24-well culture plates, T-flasks, polyolefin gas-permeable ... More
Long-term cultivation of functional human macrophages in Teflon dishes with serum-free media.
Authors Helinski E H; Bielat K L; Ovak G M; Pauly J L;
JournalJ Leukoc Biol
PubMed ID3404068
Reported herein are the results of studies demonstrating the utility of a chemically defined, serum-free medium designated as AIM-V (GIBCO) for the long-term (greater than 2 weeks) cultivation of functionally-defined human macrophages. The AIM-V medium is a mixture of HEPES-buffered Dulbecco's Modified Eagle Medium and Ham's Nutrient Mixture F12 that ... More
Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation.
AuthorsIsachenko V, Lapidus I, Isachenko E, Krivokharchenko A, Kreienberg R, Woriedh M, Bader M, Weiss JM
JournalReproduction
PubMed ID19439559
Cryopreservation as a process can be divided into two methods: conventional freezing and vitrification. The high effectiveness of vitrification in comparison with conventional freezing for human oocytes and embryos is shown, whereas data on human ovarian tissue are limited. The aim of this study was to compare the safety and ... More
Study of tumor-infiltrating lymphocytes for adoptive therapy of renal cell carcinoma (RCC) and metastatic melanoma: sequential proliferation of cytotoxic natural killer and noncytotoxic T cells in RCC.
Authors Hayakawa K; Salmeron M A; Parkinson D R; Markowitz A B; von Eschenbach A C; Legha S S; Balch C M; Ross M I; Augustus L B; Itoh K;
JournalJ Immunother
PubMed ID1790139
We investigated the immunological properties of interleukin-2 (IL-2)-activated tumor-infiltrating lymphocytes (TIL), which were used for adoptive therapy of renal cell carcinoma (RCC) (seven patients) by focusing on natural killer (NK) cells, and metastatic melanoma (six patients) by focusing on cytotoxic T lymphocytes. TIL from five of seven cases proliferated well ... More
B-Cell Responses to Intramuscular Administration of a Bivalent Virus-Like Particle Human Norovirus Vaccine.
AuthorsRamani S, Neill FH, Ferreira J, Treanor JJ, Frey SE, Topham DJ, Goodwin RR, Borkowski A, Baehner F, Mendelman PM, Estes MK, Atmar RL
JournalClin Vaccine Immunol
PubMed ID28249841
'Human noroviruses (HuNoVs) are a leading cause of acute gastroenteritis worldwide. A virus-like particle (VLP) candidate vaccine induces the production of serum histo-blood group antigen (HBGA)-blocking antibodies, the first identified correlate of protection from HuNoV gastroenteritis. Recently, virus-specific IgG memory B cells were identified to be another potential correlate of ... More
Identification and characterization of enhancer agonist human cytotoxic T-cell epitopes of the human papillomavirus type 16 (HPV16) E6/E7.
AuthorsTsang KY, Fantini M, Fernando RI, Palena C, David JM, Hodge JW, Gabitzsch ES, Jones FR, Schlom J
JournalVaccine
PubMed ID28389098
'Human papillomavirus (HPV) is associated with the etiology of cervical carcinoma, head and neck squamous cell carcinoma, and several other cancer types. Vaccines directed against HPV virus-like particles and coat proteins have been extremely successful in the prevention of cervical cancer through the activation of host HPV-specific antibody responses; however, ... More
A Rapid Cell Expansion Process for Production of Engineered Autologous CAR-T Cell Therapies.
AuthorsLu TL, Pugach O, Somerville R, Rosenberg SA, Kochendefer JN, Better M, Feldman SA
JournalHum Gene Ther Methods
PubMed ID27897048
The treatment of B-cell malignancies by adoptive cell transfer (ACT) of anti-CD19 chimeric antigen receptor T cells (CD19 CAR-T) has proven to be a highly successful therapeutic modality in several clinical trials.(1-6) The anti-CD19 CAR-T cell production method used to support initial trials relied on numerous manual, open process steps, ... More
N-acetyl cysteine protects anti-melanoma cytotoxic T cells from exhaustion induced by rapid expansion via the downmodulation of Foxo1 in an Akt-dependent manner.
AuthorsScheffel MJ, Scurti G, Wyatt MM, Garrett-Mayer E, Paulos CM, Nishimura MI, Voelkel-Johnson C
JournalCancer Immunol Immunother
PubMed ID29396710
Therapeutic outcomes for adoptive cell transfer (ACT) therapy are constrained by the quality of the infused T cells. The rapid expansion necessary to obtain large numbers of cells results in a more terminally differentiated phenotype with decreased durability and functionality. N-acetyl cysteine (NAC) protects against activation-induced cell death (AICD) and ... More