Nuclear tumor necrosis factor receptor-associated factor 6 in lymphoid cells negatively regulates c-Myb-mediated transactivation through small ubiquitin-related modifier-1 modification.
AuthorsPham LV, Zhou HJ, Lin-Lee YC, Tamayo AT, Yoshimura LC, Fu L, Darnay BG, Ford RJ,
JournalJ Biol Chem
PubMed ID18093978
'Tumor necrosis factor receptor-associated factor 6 (TRAF6) is an adaptor/scaffold protein that mediates several important signaling pathways, including the tumor necrosis factor-R:NF-kappaB pathway, involved in immune surveillance, inflammation, etc. Because most studies of TRAF6 function have focused primarily on its role as an adaptor molecule in signaling pathways in the ... More
The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes.
AuthorsLin CW, Engelman A,
JournalJ Virol
PubMed ID12663813
'Retroviral integration in vivo is mediated by preintegration complexes (PICs) derived from infectious virions. In addition to the integrase enzyme and cDNA substrate, PICs contain a variety of viral and host cell proteins. Whereas two different cell proteins, high-mobility group protein A1 (HMGA1) and the barrier-to-autointegration factor (BAF), were identified ... More
Constitutive expression of functionally active cyclin-dependent kinases and their binding partners suggests noncanonical functions of cell cycle regulators in differentiated neurons.
AuthorsSchmetsdorf S, Gärtner U, Arendt T,
JournalCereb Cortex
PubMed ID17050646
'Neurodegeneration in Alzheimer''s disease and various experimental lesion paradigms are associated with an unscheduled upregulation of cell cycle-related proteins, indicating a link between cell cycle reactivation and neuronal death. Recent evidence, however, suggests that at least some of the canonical cell cycle regulators are constitutively expressed in differentiated neurons of ... More
A DOUBLETIME kinase binding domain on the Drosophila PERIOD protein is essential for its hyperphosphorylation, transcriptional repression, and circadian clock function.
AuthorsKim EY, Ko HW, Yu W, Hardin PE, Edery I,
JournalMol Cell Biol
PubMed ID17452449
'A common feature of animal circadian clocks is the progressive phosphorylation of PERIOD (PER) proteins from hypo- to hyperphosphorylated species, events that are highly dependent on casein kinase 1 epsilon (termed DOUBLETIME [DBT] in Drosophila melanogaster) and necessary for normal clock progression. Drosophila PER (dPER) functions in the negative limb ... More
Parallel activation of phosphatidylinositol 4-kinase and phospholipase C by the extracellular calcium-sensing receptor.
AuthorsHuang C, Handlogten ME, Miller RT,
JournalJ Biol Chem
PubMed ID11907035
'The calcium-sensing receptor (CaR) is a G protein-coupled receptor that regulates physiological processes including Ca(2+) metabolism, Na(+), Cl(-), K(+), and H(2)0 balance, and the growth of some epithelial cells through diverse signaling pathways. Although many effects of CaR are mediated by the heterotrimeric G proteins Galpha(q) and Galpha(i), not all ... More
Nuclear CD40 interacts with c-Rel and enhances proliferation in aggressive B-cell lymphoma.
AuthorsZhou HJ, Pham LV, Tamayo AT, Lin-Lee YC, Fu L, Yoshimura LC, Ford RJ,
JournalBlood
PubMed ID17567982
CD40 is an integral plasma membrane-associated member of the TNF receptor family that has recently been shown to also reside in the nucleus of both normal B cells and large B-cell lymphoma (LBCL) cells. However, the physiological function of CD40 in the B-cell nucleus has not been examined. In this ... More
Regulation of Op18 during spindle assembly in Xenopus egg extracts.
AuthorsBudde PP, Kumagai A, Dunphy WG, Heald R,
JournalJ Cell Biol
PubMed ID11285281
Oncoprotein 18 (Op18) is a microtubule-destabilizing protein that is negatively regulated by phosphorylation. To evaluate the role of the three Op18 phosphorylation sites in Xenopus (Ser 16, 25, and 39), we added wild-type Op18, a nonphosphorylatable triple Ser to Ala mutant (Op18-AAA), and to mimic phosphorylation, a triple Ser to ... More
Dynamic changes in transcription factor complexes during erythroid differentiation revealed by quantitative proteomics.
AuthorsBrand M, Ranish JA, Kummer NT, Hamilton J, Igarashi K, Francastel C, Chi TH, Crabtree GR, Aebersold R, Groudine M,
JournalNat Struct Mol Biol
PubMed ID14718926
During erythroid differentiation, beta-globin gene expression is regulated by the locus control region (LCR). The transcription factor NF-E2p18/MafK binds within this region and is essential for beta-globin expression in murine erythroleukemia (MEL) cells. Here we use the isotope-coded affinity tag (ICAT) technique of quantitative mass spectrometry to compare proteins interacting ... More
Resolving cadherin interactions and binding cooperativity at the single-molecule level.
AuthorsZhang Y, Sivasankar S, Nelson WJ, Chu S,
JournalProc Natl Acad Sci U S A
PubMed ID19114658
The cadherin family of Ca(2+)-dependent cell adhesion proteins are critical for the morphogenesis and functional organization of tissues in multicellular organisms, but the molecular interactions between cadherins that are at the core of cell-cell adhesion are a matter of considerable debate. A widely-accepted model is that cadherins adhere in 3 ... More
A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.
AuthorsBerg MG, Wan L, Younis I, Diem MD, Soo M, Wang C, Dreyfuss G
JournalMol Cell Biol
PubMed ID22252314
Despite intensive research, there are very few reagents with which to modulate and dissect the mRNA splicing pathway. Here, we describe a novel approach to identify such tools, based on detection of the exon junction complex (EJC), a unique molecular signature that splicing leaves on mRNAs. We developed a high-throughput, ... More