Resolving cadherin interactions and binding cooperativity at the single-molecule level.
AuthorsZhang Y, Sivasankar S, Nelson WJ, Chu S,
JournalProc Natl Acad Sci U S A
PubMed ID19114658
The cadherin family of Ca(2+)-dependent cell adhesion proteins are critical for the morphogenesis and functional organization of tissues in multicellular organisms, but the molecular interactions between cadherins that are at the core of cell-cell adhesion are a matter of considerable debate. A widely-accepted model is that cadherins adhere in 3 ... More
A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.
AuthorsBerg MG, Wan L, Younis I, Diem MD, Soo M, Wang C, Dreyfuss G
JournalMol Cell Biol
PubMed ID22252314
Despite intensive research, there are very few reagents with which to modulate and dissect the mRNA splicing pathway. Here, we describe a novel approach to identify such tools, based on detection of the exon junction complex (EJC), a unique molecular signature that splicing leaves on mRNAs. We developed a high-throughput, ... More