An examination of the utility of a nuclear DNA/mitochondrial DNA duplex qPCR assay to assess surface decontamination of hair.
Authors:Date-Chong M, Buoncristiani MR, Aceves M, Orrego C
Journal:Forensic Sci Int Genet
PubMed ID:23582697
'The goal of this study was to compare two commonly used methods for the surface decontamination of human hair shafts, and to evaluate the use of a duplex real-time qPCR assay to assess decontamination effectiveness for the purpose of mitochondrial DNA typing. Hair shafts of known mitochondrial DNA haplotype were ... More
Contact conductivity detection in poly(methyl methacylate)-based microfluidic devices for analysis of mono- and polyanionic molecules.
Authors: Galloway Michelle; Stryjewski Wieslaw; Henry Alyssa; Ford Sean M; Llopis Shawn; McCarley Robin L; Soper Steven A;
Journal:Anal Chem
PubMed ID:12038768
'An on-column contact conductivity detector was developed for the analysis of various mono- and polyanionic compounds separated by electrophoresis chips fabricated in poly(methyl methacrylate) (PMMA) using hot embossing techniques from Ni electroforms. The detector consisted of a pair of Pt wires (127 microm diameter) with an end-to-end spacing of approximately ... More
MultiPSQ: a software solution for the analysis of diagnostic n-plexed pyrosequencing reactions.
Authors:Dabrowski PW, Schröder K, Nitsche A
Journal:PLoS One
PubMed ID:23555882
Pyrosequencing can be applied for Single-Nucleotide-Polymorphism (SNP)-based pathogen typing or for providing sequence information of short DNA stretches. However, for some pathogens molecular typing cannot be performed relying on a single SNP or short sequence stretch, necessitating the consideration of several genomic regions. A promising rapid approach is the simultaneous ... More
The use of the MegaBACE for sequencing and genotype analysis.
Authors:Burger PA
Journal:Methods Mol Biol
PubMed ID:23546794
Despite the advent of next generation sequencing techniques, which provide access to an enormous amount of genomic information in a relatively short time, the conventional Sanger sequencing and microsatellite genotyping analyses present a straightforward method to answer clearly defined questions in population genetics, phylogeography, or forensics. The MegaBACE is a ... More
The Histone Deacetylase Inhibitor Sodium Butyrate Promotes Cell Death and Differentiation and Reduces Neurosphere Formation in Human Medulloblastoma Cells.
Authors:Nör C, Sassi FA, de Farias CB, Schwartsmann G, Abujamra AL, Lenz G, Brunetto AL, Roesler R
Journal:Mol Neurobiol
PubMed ID:23516101
Increasing evidence suggests that alterations in epigenetic mechanisms regulating chromatin state play a role in the pathogenesis of medulloblastoma (MB), the most common malignant brain tumor of childhood. Histone deacetylase (HDAC) inhibitors, which increase chromatin relaxation, have been shown to display anticancer activities. Here we show that the HDAC inhibitor ... More