Low DNA Mass 分子量标准
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Low DNA Mass 分子量标准
Invitrogen™

Low DNA Mass 分子量标准

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Invitrogen Low DNA Mass 分子量标准设计用于 100 bp 至 2,000 bp了解更多信息
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货号数量
1006801323.5 μg
货号 10068013
价格(CNY)
796.00
飞享价
Ends: 31-Dec-2025
2,536.00
共减 1,740.00 (69%)
Each
添加至购物车
数量:
23.5 μg
请求批量或定制报价
价格(CNY)
796.00
飞享价
Ends: 31-Dec-2025
2,536.00
共减 1,740.00 (69%)
Each
添加至购物车
Invitrogen Low DNA Mass 分子量标准设计用于 100 bp 至 2,000 bp 内双链 DNA 片段的大小测定和近似定量。Low DNA Mass 分子量标准由 6 个层析纯化的个体 DNA 片段组成。

Low DNA Mass 分子量标准非常适合利用 1–2% 琼脂糖凝胶进行 DNA 片段分离。

Low DNA Mass 分子量标准的特点:
明显、清晰的条带—层析纯化片段可获得一致和可靠的结果
便利—已经预混了 10X BlueJuice 凝胶上样缓冲液,便于追踪样品 DNA 迁移
精确—可获得每条带中的 DNA 精确量

产品用途
溴化乙锭或 SYBR Safe 染色后,可在 1–2% 琼脂糖凝胶上观测到双链 DNA 分子量标准。该分子量标准适用于强度递增的 DNA 条带。根据每条带中的 DNA 精确量,可以对 DNA 样品近似定量。

仅供科研使用。不可用于诊断程序。
规格
最大浓度0.1175 μg/μL
凝胶兼容性琼脂糖凝胶
环保功能绿色可持续包装
反应次数50应用
产品类型DNA 分子量标准品
数量23.5 μg
即用型
上样容量1 mL
运输条件经批准可在室温或干冰上运输
技术层析纯化的个体 DNA 片段
容积(公制)200 µL
凝胶类型琼脂糖
大小范围100 bp-2,000 bp
Unit SizeEach
内容与储存
• 200 µL Low DNA Mass 分子量标准
• 1 mL10X BlueJuice 凝胶上样缓冲液

储存于 -20°C。

常见问题解答 (FAQ)

为什么我的分子量ladder 的DNA条带出现了弥散现象?

这里是一些可能导致条带弥散的原因:

•DNA被降解。请避免DNA标准品被核酸酶污染。
•凝胶内上样了过多的DNA。减少凝胶内的DNA量。
•DNA被蛋白污染。电泳前使用苯酚抽提除去蛋白。
•对于小分子量DNA,条带可能会在染色时扩散。在电泳之前加入溴化乙锭(EB)。
•对于放射性标记的DNA,标记是使用切口平移进行的。使用T4 DNA聚合酶对DNA进行置换合成来标记,或者使用T4多核苷酸激酶进行5’末端标记。
•使用了不合适的电泳条件。电泳电压不能超过~20 V/cm,电泳时保持温度<30°C,检查电泳缓冲液是否具有足够的缓冲能力。
•DNA含盐太多。电泳前使用乙醇沉淀去除过多的盐。

我的DNA ladder出现异常迁移。这是为什么?

如果marker被加热了,会出现这种情况。请确保DNA ladder在使用前没有被加热过。

我需要进行DNA定量。你们建议使用哪种梯度标准品?

我们的低分子量DNA Mass 梯度标准品(货号10068013)和高分子量DNA Mass梯度标准品(货号10496016)专用于定量评估凝胶中的DNA质量。

TrackIt DNA梯度标准品与其他DNA梯度标准品有何区别?

TrackIt DNA梯度标准品的样品缓冲液中含有2种示踪染料,可作为观察凝胶电泳进程的视觉指示分子,并能指示何时达到最大分辨率。示踪染料不会遮盖梯度标准品中的DNA条带,因为染料会跑出梯度标准品中大部分DNA条带的范围。
TrackIt蓝绿色/橙黄色上样缓冲液的配方含有独特的示踪染料,二甲苯蓝FF和橙黄G。我们推荐将TrackIt蓝绿色/橙黄色上样缓冲液用于10bp至1kb间的DNA片段。
TrackIt蓝绿色/黄色上样缓冲液含有独特的示踪染料,二甲苯蓝FF和柠檬黄。我们推荐将TrackIt蓝绿色/黄色上样缓冲液用于100bp至10kb间的DNA片段。二甲苯蓝FF的分子量为638.6,橙黄G为452.4,柠檬黄为534.4。
备注:不推荐将TrackIt DNA 梯度标准品用于聚丙烯酰氨凝胶,并且不推荐用于定量分析。

Can I know the sequences of Invitrogen DNA ladders?

Sequences of Invitrogen DNA and RNA ladders are proprietary.

引用和文献 (8)

引用和文献
Abstract
An examination of the utility of a nuclear DNA/mitochondrial DNA duplex qPCR assay to assess surface decontamination of hair.
Authors:Date-Chong M, Buoncristiani MR, Aceves M, Orrego C
Journal:Forensic Sci Int Genet
PubMed ID:23582697
'The goal of this study was to compare two commonly used methods for the surface decontamination of human hair shafts, and to evaluate the use of a duplex real-time qPCR assay to assess decontamination effectiveness for the purpose of mitochondrial DNA typing. Hair shafts of known mitochondrial DNA haplotype were ... More
Contact conductivity detection in poly(methyl methacylate)-based microfluidic devices for analysis of mono- and polyanionic molecules.
Authors: Galloway Michelle; Stryjewski Wieslaw; Henry Alyssa; Ford Sean M; Llopis Shawn; McCarley Robin L; Soper Steven A;
Journal:Anal Chem
PubMed ID:12038768
'An on-column contact conductivity detector was developed for the analysis of various mono- and polyanionic compounds separated by electrophoresis chips fabricated in poly(methyl methacrylate) (PMMA) using hot embossing techniques from Ni electroforms. The detector consisted of a pair of Pt wires (127 microm diameter) with an end-to-end spacing of approximately ... More
MultiPSQ: a software solution for the analysis of diagnostic n-plexed pyrosequencing reactions.
Authors:Dabrowski PW, Schröder K, Nitsche A
Journal:PLoS One
PubMed ID:23555882
Pyrosequencing can be applied for Single-Nucleotide-Polymorphism (SNP)-based pathogen typing or for providing sequence information of short DNA stretches. However, for some pathogens molecular typing cannot be performed relying on a single SNP or short sequence stretch, necessitating the consideration of several genomic regions. A promising rapid approach is the simultaneous ... More
The use of the MegaBACE for sequencing and genotype analysis.
Authors:Burger PA
Journal:Methods Mol Biol
PubMed ID:23546794
Despite the advent of next generation sequencing techniques, which provide access to an enormous amount of genomic information in a relatively short time, the conventional Sanger sequencing and microsatellite genotyping analyses present a straightforward method to answer clearly defined questions in population genetics, phylogeography, or forensics. The MegaBACE is a ... More
The Histone Deacetylase Inhibitor Sodium Butyrate Promotes Cell Death and Differentiation and Reduces Neurosphere Formation in Human Medulloblastoma Cells.
Authors:Nör C, Sassi FA, de Farias CB, Schwartsmann G, Abujamra AL, Lenz G, Brunetto AL, Roesler R
Journal:Mol Neurobiol
PubMed ID:23516101
Increasing evidence suggests that alterations in epigenetic mechanisms regulating chromatin state play a role in the pathogenesis of medulloblastoma (MB), the most common malignant brain tumor of childhood. Histone deacetylase (HDAC) inhibitors, which increase chromatin relaxation, have been shown to display anticancer activities. Here we show that the HDAC inhibitor ... More