Fetal Bovine Serum, qualified, heat inactivated, Australia - FAQs

Q: When should I use heat-inactivated serum?

Heat-inactivation of serum inactivates the complements in the serum. In general, it is not necessary to use heat-inactivated serum to culture most cell types. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications. Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center .

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3 个常见问题解答

When should I use heat-inactivated serum?

Heat-inactivation of serum inactivates the “complements” in the serum. In general, it is not necessary to use heat-inactivated serum to culture most cell types. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I would like to order heat-inactivated FBS. What conditions do you use for heat-inactivation of FBS during the manufacturing process?

Serum is heat inactivated by heating at 56 degrees C in a water bath for 30 min, swirling the bottle approximately every 10 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.