Nucleotide incorporation by human DNA polymerase gamma opposite benzo[a]pyrene and benzo[c]phenanthrene diol epoxide adducts of deoxyguanosine and deoxyadenosine.
'Mitochondria are major cellular targets of benzo[a]pyrene (BaP), a known carcinogen that also inhibits mitochondrial proliferation. Here, we report for the first time the effect of site-specific N2-deoxyguanosine (dG) and N6-deoxyadenosine (dA) adducts derived from BaP 7,8-diol 9,10-epoxide (BaP DE) and dA adducts from benzo[c]phenanthrene 3,4-diol 1,2-epoxide (BcPh DE) on ... More
Instability of a multiple copy enhancer in plasmid vectors: practical considerations.
AuthorsDischer D J; Bishopric N H; Hernandez O M; Webster K A;
JournalBiotechniques
PubMed ID10376132
Working with a construct containing 4 tandem copies of a 60-mer (consistancy of the erythropoietin hypoxin-inducible enhancer element with linker cloning sequences), researchers show the effects of vector choice and E. coli host choice on deletion AND amplification events on this repeat. ... More