Silencing of Transcription of the Human Luteinizing Hormone Receptor Gene by Histone Deacetylase-mSin3A Complex.
Authors Zhang Ying; Dufau Maria L;
JournalJ Biol Chem
PubMed ID12091390
'Modification of chromatin structure by histone acetylases and deacetylases is an important mechanism in modulation of eukaryotic gene transcription. The present study investigated regulation of the human luteinizing hormone receptor (hLHR) gene by histone deacetylases. Inhibition of histone deacetylases (HDACs) by trichostatin A (TSA) increased hLHR promoter activity by 40-fold ... More
An endogenous Drosophila receptor for glycans bearing alpha 1,3-linked core fucose residues.
Authors Bouyain Samuel; Silk Nicholas J; Fabini Gustáv; Drickamer Kurt;
JournalJ Biol Chem
PubMed ID11960993
'The genome of Drosophila melanogaster encodes several proteins that are predicted to contain Ca(2+)-dependent, C-type carbohydrate-recognition domains. The CG2958 gene encodes a protein containing 359 amino acid residues. Analysis of the CG2958 sequence suggests that it consists of an N-terminal domain found in other Drosophila proteins, a middle segment that ... More
Basic Residues in Azurocidin/HBP Contribute to Both Heparin Binding and Antimicrobial Activity.
'Azurocidin/CAP37/HBP is an antimicrobial and chemotactic protein that is part of the innate defenses of human neutrophils. In addition, azurocidin is an inactive serine protease homolog with binding sites for diverse ligands including heparin and the bovine pancreatic trypsin inhibitor (BPTI). The structure of the protein reveals a highly cationic ... More
Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends.
AuthorsTrujillo KM, Roh DH, Chen L, Van Komen S, Tomkinson A, Sung P,
JournalJ Biol Chem
PubMed ID14522986
'Saccharomyces cerevisiae Rad50, Mre11, and Xrs2 proteins are involved in homologous recombination, non-homologous end-joining, DNA damage checkpoint signaling, and telomere maintenance. These proteins form a stable complex that has nuclease, DNA binding, and DNA end recognition activities. Of the components of the Rad50.Mre11.Xrs2 complex, Xrs2 is the least characterized. The ... More
Repression of Na,K-ATPase beta1-subunit by the transcription factor snail in carcinoma.
AuthorsEspineda CE, Chang JH, Twiss J, Rajasekaran SA, Rajasekaran AK,
JournalMol Biol Cell
PubMed ID14699059
'The Na,K-ATPase consists of two essential alpha- and beta-subunits and regulates the intracellular Na+ and K+ homeostasis. Although the alpha-subunit contains the catalytic activity, it is not active without functional beta-subunit. Here, we report that poorly differentiated carcinoma cell lines derived from colon, breast, kidney, and pancreas show reduced expression ... More
High-affinity salicylic acid-binding protein 2 is required for plant innate immunity and has salicylic acid-stimulated lipase activity.
AuthorsKumar D, Klessig DF,
JournalProc Natl Acad Sci U S A
PubMed ID14673096
'Salicylic acid (SA) is a critical hormone for signaling innate immunity in plants. Here we present the purification and characterization of SA-binding protein 2 (SABP2), a tobacco protein that is present in low abundance and specifically binds SA with high affinity. Sequence analysis predicted that SABP2 is a lipase belonging ... More
A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase.
'The modified nucleoside 1-methyladenosine (m(1)A) is found in the T-loop of many tRNAs from organisms belonging to the three domains of life (Eukaryota, Bacteria, Archaea). In the T-loop of eukaryotic and bacterial tRNAs, m(1)A is present at position 58, whereas in archaeal tRNAs it is present at position(s) 58 and/or ... More
Nucleotide incorporation by human DNA polymerase gamma opposite benzo[a]pyrene and benzo[c]phenanthrene diol epoxide adducts of deoxyguanosine and deoxyadenosine.
'Mitochondria are major cellular targets of benzo[a]pyrene (BaP), a known carcinogen that also inhibits mitochondrial proliferation. Here, we report for the first time the effect of site-specific N2-deoxyguanosine (dG) and N6-deoxyadenosine (dA) adducts derived from BaP 7,8-diol 9,10-epoxide (BaP DE) and dA adducts from benzo[c]phenanthrene 3,4-diol 1,2-epoxide (BcPh DE) on ... More
Induction of cIAP-2 in human colon cancer cells through PKC delta/NF-kappa B.
AuthorsWang Q, Wang X, Evers BM,
JournalJ Biol Chem
PubMed ID14527959
'Activation of protein kinase C (PKC) prevents apoptosis in certain cells; however, the mechanisms are largely unknown. Inhibitors of apoptosis (IAP) family members, including NAIP, cIAP-1, cIAP-2, XIAP/hILP, survivin, and BRUCE, block apoptosis by binding and potently inhibiting caspases. Activation of NF-kappa B contributes to cIAP-2 induction; however, the cellular ... More
The novel WD-repeat protein MORG1 acts as a molecular scaffold for HIF prolyl-hydroxylase 3 (PHD3).
AuthorsHopfer U, Hopfer H, Jablonski K, Stahl RA, Wolf G,
JournalJ Biol Chem
PubMed ID16407229
'Hypoxia-inducible factor-1 (HIF-1), a transcriptional complex composed of an oxygen-sensitive alpha- and a beta-subunit, plays a pivotal role in cellular adaptation to low oxygen availability. Under normoxia, the alpha-subunit of HIF-1 is hydroxylated by a family of prolyl hydroxylases (PHDs) and consequently targeted for proteasomal degradation. Three different prolyl hydroxylases ... More
IL-4 and IL-13 up-regulate intestinal trefoil factor expression: requirement for STAT6 and de novo protein synthesis.
AuthorsBlanchard C, Durual S, Estienne M, Bouzakri K, Heim MH, Blin N, Cuber JC,
JournalJ Immunol
PubMed ID15004182
'The development of intestinal goblet cell hyperplasia/hypertrophy during nematode infection involves the Th2 cytokines IL-4 and IL-13 via STAT6 activation. This is thought to play an important role in host protective immunity against the infection. In this study we demonstrate that IL-4 and IL-13 up-regulate the specific goblet cell product ... More
Disputed maternity leading to identification of tetragametic chimerism.
Authors Yu Neng; Kruskall Margot S; Yunis Juan J; Knoll Joan H M; Uhl Lynne; Alosco Sharon; Ohashi Marina; Clavijo Olga; Husain Zaheed; Yunis Emilio J; Yunis Jorge J; Yunis Edmond J;
JournalN Engl J Med
PubMed ID12015394
Complex DNA melting profiles of small PCR products revealed using SYBR Green I.
AuthorsLi W, Xi B, Yang W, Hawkins M, Schubart UK,
JournalBiotechniques
PubMed ID14579734
None
Instability of a multiple copy enhancer in plasmid vectors: practical considerations.
AuthorsDischer D J; Bishopric N H; Hernandez O M; Webster K A;
JournalBiotechniques
PubMed ID10376132
Working with a construct containing 4 tandem copies of a 60-mer (consistancy of the erythropoietin hypoxin-inducible enhancer element with linker cloning sequences), researchers show the effects of vector choice and E. coli host choice on deletion AND amplification events on this repeat. ... More
Identification of cytoplasmic domains of hVPAC1 receptor required for activation of adenylyl cyclase. Crucial role of two charged amino acids strictly conserved in class II G protein-coupled receptors.
AuthorsCouvineau A, Lacapere JJ, Tan YV, Rouyer-Fessard C, Nicole P, Laburthe M,
JournalJ Biol Chem
PubMed ID12690118
The VPAC1 receptor mediates the action of two neuropeptides, vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide. It is a class II G protein-coupled receptor-activating adenylyl cyclase (AC). The role of the N-terminal extracellular domain of hVPAC1 receptor for VIP binding is now established (Laburthe, M., Couvineau, A. and ... More
Genetic complementation between mutant b subunits in F1F0 ATP synthase.
AuthorsGrabar TB, Cain BD,
JournalJ Biol Chem
PubMed ID15159387
In Escherichia coli, a parallel homodimer of identical b subunits constitutes the peripheral stalk of F1F0 ATP synthase. Although the two b subunits have long been viewed as a single functional unit, the asymmetric nature of the enzyme complex suggested that the functional roles of each b subunit should not ... More
Novel isoforms of the sodium channels Nav1.8 and Nav1.5 are produced by a conserved mechanism in mouse and rat.
AuthorsKerr NC, Holmes FE, Wynick D,
JournalJ Biol Chem
PubMed ID15047701
The voltage-gated sodium channel Nav1.8 is only expressed in subsets of neurons in dorsal root ganglia (DRG), trigeminal and nodose ganglia. We have isolated mouse partial-length Nav1.8 cDNA clones spanning exon 17 sequence which have 17 nucleotide substitutions and 12 predicted amino acid differences from the published sequence. The absence ... More
Spermidine but not spermine is essential for hypusine biosynthesis and growth in Saccharomyces cerevisiae: Spermine is converted to spermidine in vivo by the FMS1-amine oxidase.
AuthorsChattopadhyay MK, Tabor CW, Tabor H,
JournalProc Natl Acad Sci U S A
PubMed ID14617780
In our earlier work we showed that either spermidine or spermine could support the growth of spe2Delta or spe3Delta polyamine-requiring mutants, but it was unclear whether the cells had a specific requirement for either of these amines. In the current work, we demonstrate that spermidine is specifically required for the ... More
The UL12.5 gene product of herpes simplex virus type 1 exhibits nuclease and strand exchange activities but does not localize to the nucleus.
AuthorsReuven NB, Antoku S, Weller SK,
JournalJ Virol
PubMed ID15078942
The herpes simplex virus type 1 (HSV-1) alkaline nuclease, encoded by the UL12 gene, plays an important role in HSV-1 replication, as a null mutant of UL12 displays a severe growth defect. Although the precise in vivo role of UL12 has not yet been determined, several in vitro activities have ... More
Efficient intracellular assembly of papillomaviral vectors.
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of transducing reporter plasmids into mammalian cells are technically demanding and relatively low-yield. In this report, we describe a simple 293 cell transfection method for efficient ... More
Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.
Authors Stemmer W P; Crameri A; Ha K D; Brennan T M; Heyneker H L;
JournalGene
PubMed ID7590320
Here, we describe assembly PCR as a method for the synthesis of long DNA sequences from large numbers of oligodeoxyribonucleotides (oligos). The method, which is derived from DNA shuffling [Stemmer, Nature 370 (1994a) 389-391], does not rely on DNA ligase but instead relies on DNA polymerase to build increasingly longer ... More
Molecular cloning and induction of bovine prostaglandin E synthase by gonadotropins in ovarian follicles prior to ovulation in vivo.
Prostaglandin E(2) (PGE(2)) is thought to be an ultimate prostaglandin effector during the ovulatory process, and the objectives of this study were to clone bovine PGE synthase (PGES) and to characterize its regulation by gonadotropins in preovulatory follicles in vivo. The bovine PGES complementary DNA (cDNA) was shown to contain ... More
The Tetraspan Protein Epithelial Membrane Protein-2 Interacts with beta 1 Integrins and Regulates Adhesion.
The growth arrest-specific-3 (GAS3)/PMP22 proteins are members of the four-transmembrane (tetraspan) superfamily. Although the function of these proteins is poorly understood, GAS3/PMP22 proteins have been implicated in the control of growth and progression of certain cancers. Epithelial membrane protein-2 (EMP2), a GAS3/PMP22 family member, was recently identified as a putative ... More
Mechanistic role of residue Gln151 in error prone DNA synthesis by human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT). Pre-steady state kinetic study of the Q151N HIV-1 RT mutant with increased fidelity.
Authors Weiss Kellie K; Bambara Robert A; Kim Baek;
JournalJ Biol Chem
PubMed ID11927582
It has previously been reported that mutations in the Gln(151) residue of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) greatly enhance RT fidelity. In this study, we employed pre-steady state kinetic assays to elucidate the mechanistic role of residue Gln(151) in highly error prone DNA synthesis by HIV-1 ... More