A model for melanosome biogenesis based on the purification and analysis of early melanosomes.
Authors Kushimoto T; Basrur V; Valencia J; Matsunaga J; Vieira W D; Ferrans V J; Muller J; Appella E; Hearing V J;
JournalProc Natl Acad Sci U S A
PubMed ID11526213
Melanosome biogenesis and function were studied after purification of early stage melanosomes and characterization of specific proteins sorted to that organelle. Melanosomes were isolated from highly pigmented human MNT1 melanoma cells after disruption and initial separation by sucrose density gradient centrifugation. Low-density sucrose fractions were found by electron microscopy to ... More