The CesA gene family of barley. Quantitative analysis of transcripts reveals two groups of co-expressed genes.
AuthorsBurton RA, Shirley NJ, King BJ, Harvey AJ, Fincher GB,
JournalPlant Physiol
PubMed ID14701917
Sequence data from cDNA and genomic clones, coupled with analyses of expressed sequence tag databases, indicate that the CesA (cellulose synthase) gene family from barley (Hordeum vulgare) has at least eight members, which are distributed across the genome. Quantitative polymerase chain reaction has been used to determine the relative abundance ... More
Targeting and functional role of N-RAP, a nebulin-related LIM protein, during myofibril assembly in cultured chick cardiomyocytes.
Authors Carroll S L; Herrera A H; Horowits R;
JournalJ Cell Sci
PubMed ID11739655
Targeting and functional effects of N-RAP domains were studied by expression as GFP-tagged fusion proteins in cultured embryonic chick cardiomyocytes. GFP-tagged N-RAP was targeted to myofibril precursors, myofibril ends and cell contacts, expression patterns that are similar to endogenous N-RAP. The GFP-tagged N-RAP LIM domain (GFP-N-RAP-LIM) was targeted to the ... More
Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters.
We cloned two cDNAs encoding proton/amino acid cotransporters, designated as mPAT1 and mPAT2, from murine tissues. They were identified by sequence similarity to the amino acid/auxin permease family member of lower eukaryotes. We functionally characterized both transporters by flux studies and electrophysiology after expression in Xenopus laevis oocytes. Both mPAT1 ... More