Alternative splicing of the primary transcript generates heterogeneity within the products of the gene for Bombyx mori chitinase.
AuthorsAbdel-Banat BM, Koga D.
JournalJ Biol Chem
PubMed ID12045191
'The gene of chitinase in the silkworm, Bombyx mori, generates four mRNA products by alternative splicing. Nucleotide sequences of the entire gene for chitinase and respective cDNAs demonstrate that the pre-mRNA undergoes alternative splicing at both the 5'' and 3'' regions. At the 5'' region, the pre-mRNA experienced differential splicing ... More
The CesA gene family of barley. Quantitative analysis of transcripts reveals two groups of co-expressed genes.
AuthorsBurton RA, Shirley NJ, King BJ, Harvey AJ, Fincher GB,
JournalPlant Physiol
PubMed ID14701917
Sequence data from cDNA and genomic clones, coupled with analyses of expressed sequence tag databases, indicate that the CesA (cellulose synthase) gene family from barley (Hordeum vulgare) has at least eight members, which are distributed across the genome. Quantitative polymerase chain reaction has been used to determine the relative abundance ... More
Gene transcription analysis of Saccharomyces cerevisiae exposed to neocarzinostatin protein-chromophore complex reveals evidence of DNA damage, a potential mechanism of resistance, and consequences of prolonged exposure.
Authors Schaus S E; Cavalieri D; Myers A G;
JournalProc Natl Acad Sci U S A
PubMed ID11562456
The natural product neocarzinostatin (NCS), a protein-small molecule complex, exhibits potent antiproliferative activity in mammalian cells but has little apparent effect on the growth of the unicellular eukaryotic organism, Saccharomyces cerevisiae. Here, we show by whole-genome transcription profiling experiments that incubation of S. cerevisiae with NCS leads to dramatic and ... More
P instability factor: an active maize transposon system associated with the amplification of Tourist-like MITEs and a new superfamily of transposases.
Authors Zhang X; Feschotte C; Zhang Q; Jiang N; Eggleston W B; Wessler S R;
JournalProc Natl Acad Sci U S A
PubMed ID11675478
Miniature inverted-repeat transposable elements (MITEs) are widespread and abundant in both plant and animal genomes. Despite the discovery and characterization of many MITE families, their origin and transposition mechanism are still poorly understood, largely because MITEs are nonautonomous elements with no coding capacity. The starting point for this study was ... More
The mechanism for regulation of the F-actin binding activity of IQGAP1 by calcium/calmodulin.
Authors Mateer Scott C; McDaniel Amanda E; Nicolas Valerie; Habermacher Geoffrey M; Lin Mei-Jung Sun; Cromer Damond A; King Michelle E; Bloom George S;
JournalJ Biol Chem
PubMed ID11809768
IQGAP1 colocalizes with actin filaments in the cell cortex and binds in vitro to F-actin and several signaling proteins, including calmodulin, Cdc42, Rac1, and beta-catenin. It is thought that the F-actin binding activity of IQGAP1 is regulated by its reversible association with these signaling molecules, but the mechanisms have remained ... More