A PCR-based DNA fingerprinting technique: AFLP for molecular typing of bacteria.
Authors Lin J J; Kuo J; Ma J;
JournalNucleic Acids Res
PubMed ID8836198
'Amplified restriction fragment polymorphism (AFLP) is a PCR-based DNA fingerprinting technique. In AFLP analysis, bacterial genomic DNA is digested with restriction enzymes, ligated to adapters, and a subset of DNA fragments are amplified using primers containing 16 adapter defined sequences with one additional arbitrary nucleotide. Polymorphisms of different Escherichia coli ... More
Cost-effective method to synthesize fluorescently labeled DNA size standards using cloned AFLP fragments.