Invitrogen TrackIt 100 bp DNA 分子量标准为即用型产品,设计用于 100 bp 至 2,000了解更多信息
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货号
数量
10488058
50 μg
货号 10488058
价格(CNY)
2,153.00
Each
添加至购物车
数量:
50 μg
请求批量或定制报价
价格(CNY)
2,153.00
Each
添加至购物车
Invitrogen TrackIt 100 bp DNA 分子量标准为即用型产品,设计用于 100 bp 至 2,000 bp 内双链 DNA 片段的大小测定和近似定量。100 bp DNA 分子量标准由 13 个层析纯化的个体 DNA 片段组成,参比条带分别为 2000、1500 和 600 bp,便于轻松定向。
TrackIt 100 bp DNA 分子量标准非常适合利用 1–2% 琼脂糖凝胶进行 DNA 片段分离。
TrackIt 100 bp DNA 分子量标准的特点: •明显、清晰的条带—层析纯化片段可获得一致和可靠的结果,在室温条件下可保持稳定长达 6 个月 • 即用型—预混了 6X TrackIt Cyan/Orange 上样缓冲液,便于追踪样品 DNA 迁移 • 精确—可获得每条带中的 DNA 精确量
产品用途 溴化乙锭或 SYBR Safe 染色后,可在 1–2% 琼脂糖凝胶上观测到双链 DNA 分子量标准。该分子量标准设计有强度均匀的 DNA 条带,便于清楚查看每一条带。根据每条带中的 DNA 精确量,可以对 DNA 样品近似定量。
仅供科研使用。不可用于诊断程序。
规格
最大浓度0.1 μg/μL
凝胶兼容性琼脂糖凝胶
环保功能绿色可持续包装
反应次数100应用
产品线TrackIt
产品类型DNA 分子量标准品
数量50 μg
即用型是
上样容量1 mL
有效期每 12 个月
运输条件经批准可在室温或置于干冰上运输
技术层析纯化的个体 DNA 片段
容积(公制)50 μg
凝胶类型琼脂糖
大小范围100 bp-2,000 bp
Unit SizeEach
内容与储存
• 500 μL TrackIt 100 bp DNA Ladder • 1 mL 6X TrackIt Cyan/Orange Loading Buffer
Store at room temperature or at 4°C for up to 24 months. For longer periods, store at -20°C.
常见问题解答 (FAQ)
我的DNA ladder出现异常迁移。这是为什么?
如果marker被加热了,会出现这种情况。请确保DNA ladder在使用前没有被加热过。
TrackIt DNA梯度标准品与其他DNA梯度标准品有何区别?
TrackIt DNA梯度标准品的样品缓冲液中含有2种示踪染料,可作为观察凝胶电泳进程的视觉指示分子,并能指示何时达到最大分辨率。示踪染料不会遮盖梯度标准品中的DNA条带,因为染料会跑出梯度标准品中大部分DNA条带的范围。
TrackIt蓝绿色/橙黄色上样缓冲液的配方含有独特的示踪染料,二甲苯蓝FF和橙黄G。我们推荐将TrackIt蓝绿色/橙黄色上样缓冲液用于10bp至1kb间的DNA片段。
TrackIt蓝绿色/黄色上样缓冲液含有独特的示踪染料,二甲苯蓝FF和柠檬黄。我们推荐将TrackIt蓝绿色/黄色上样缓冲液用于100bp至10kb间的DNA片段。二甲苯蓝FF的分子量为638.6,橙黄G为452.4,柠檬黄为534.4。
备注:不推荐将TrackIt DNA 梯度标准品用于聚丙烯酰氨凝胶,并且不推荐用于定量分析。
Can I know the sequences of Invitrogen DNA ladders?
Sequences of Invitrogen DNA and RNA ladders are proprietary.
Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?
Invitrogen DNA ladders contain linear dsDNA fragments.
Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?
Invitrogen DNA ladders are composed of double-stranded DNA fragments only.
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