Computational prediction and experimental validation of novel markers for detection of STEC O157:H7.
AuthorsWang GQ, Zhou FF, Olman V, Su YY, Xu Y, Li F
JournalWorld J Gastroenterol
PubMed ID21528067
'To identify and assess the novel makers for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 with an integrated computational and experimental approach.'
Determination of the mode of action of enterolysin A, produced by Enterococcus faecalis B9510.
AuthorsKhan H, Flint SH, Yu PL
JournalJ Appl Microbiol
PubMed ID23639072
The current study aimed to visualize the damage caused by enterolysin A to the cells of sensitive strains and to find out cleavage site within the peptidoglycan moiety of bacterial cell walls. ... More
High isoprenoid flux Escherichia coli as a host for carotenoids production.
AuthorsSuh W
JournalMethods Mol Biol
PubMed ID22144352
A noncarotenogenic microbe E. coli was engineered for high production of carotenoids. To increase the isoprenoid flux, the chromosomal native promoters of the rate-controlling steps (dxs, idi and ispDispF) in the isoprenoid pathway were replaced with a strong bacteriophage T5 promoter (P(T5)) by using the ?-Red recombinase system in combination ... More