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Fetal Bovine Serum, qualified, heat inactivated, Brazil - FAQs

Fetal Bovine Serum, qualified, heat inactivated, Brazil - FAQs

查看更多产品信息 Fetal Bovine Serum, qualified, heat inactivated, Brazil - FAQs (10500056)

9 个常见问题解答

如果收到的FBS已经部分化冻，我还能继续使用么？

我们的研究结果显示在4°C条件下短时保存化冻的FBS，28天（之内）不会降低其生长或活性方面的效能。如需在4°C条件下将FBS保存更长时间，就需要逐案评估这一储存条件是否为必须。

我有时在血清中见到絮状沉淀。那可能是些什么物质？

多种原因可能导致FBS中出现絮状沉淀。最为常见的原因是血清中脂蛋白的变性。你可能会在血清化冻后观察到血纤蛋白——一种血清中存在的促凝蛋白。这并不会影响本品的使用性能。

如需去除絮状沉淀，请将血清移至无菌管中，以400 x g进行简短离心。之后把所得的上清液和培养基一并过滤。不要试图过滤含有絮状沉淀的血清，可能会堵塞滤器。

存放于冰箱中的FBS中为何会出现沉淀？

Gibco FBS未经预老化处理。当储存于2至8°C的条件下，血清中可能存在的各类蛋白与脂蛋白（如冷凝集素，纤维蛋白原，玻连蛋白等）会发生聚集，并且形成肉眼可见的沉淀或混浊。这并不会影响血清的使用性能。我们推荐您将FBS储存于–20°C，同时避免反复冻融。

When should I use heat-inactivated serum?

Heat-inactivation of serum inactivates the “complements” in the serum. In general, it is not necessary to use heat-inactivated serum to culture most cell types. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I would like to order heat-inactivated FBS. What conditions do you use for heat-inactivation of FBS during the manufacturing process?

Serum is heat inactivated by heating at 56 degrees C in a water bath for 30 min, swirling the bottle approximately every 10 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

If my FBS arrives partially thawed, can I still use it?

Our studies have shown that short-term storage of thawed FBS at 4 degrees C for up to 28 days causes no decrease in growth or viability performance. FBS stored at 4 degrees C for longer periods of time should be evaluated on a case-by-case basis should this storage condition be necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Sometimes I notice flocculence in serum. What could it be?

Flocculence may appear in FBS for a variety of reasons. The most common reason is the denaturation of serum lipoproteins. You may observe fibrin, one of the clot-forming proteins present in serum, after the serum has been thawed. This should not affect product performance. To remove the flocculence, transfer the serum to sterile tubes and centrifuge the material briefly at 400 x g. Then filter the resulting supernant along with your media. Do not attempt to filter serum containing flocculence, it may clog filters.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does material precipitate in FBS upon storage in the refrigerator?

Gibco FBS is not pre-aged. When stored at 2 to 8 degrees C, the possibility exists for various proteins and lipoproteins in serum (e.g., cold agglutinins, fibrinogen, vitronectin, etc.) to aggregate, and form either perceptible material or observed turbidity. This should not affect serum performance. We recommend that you store FBS at -20 degrees C and avoid repeated freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.