从多种样品类型中高效分离基因组 DNA DNAzol™ 试剂程序使用一种新型胍-去污剂裂解液,可从细胞裂解物中选择性沉淀 DNA。1 mL DNAzol™ 试剂可用于从 1–3 x 107 个细胞、0.1 mL 全血或 25–50 mg 组织中分离基因组 DNA。
快速分离基因组 DNA 且回收率较高 DNAzol™ 试剂在其 DNA 分离方案中兼具可靠性、高效性和简单性。在分离期间,生物样品在 DNAzol™ 试剂中裂解(或均质化),然后用乙醇从裂解物中沉淀基因组 DNA。乙醇洗涤后,DNA 可能溶解于水或 8 mM NaOH 中。整个程序可在 10–30 min 内完成,DNA 回收率为 70–100%。
分离的 DNA 可用于多种下游应用 使用 DNAzol™ 程序提取的基因组 DNA 适用于多种应用,包括 Southern 印迹、克隆、PCR、限制性核酸内切酶酶切和斑点印迹杂交。
仅供科研使用。不可用于诊断程序。
规格
洗脱体积Varies
最终产品类型基因组 DNA 纯化
适用于(应用)PCR、Southern 印迹、测序、克隆
高通量能力不兼容高通量应用(手动)
数量100 mL
样品类型细胞, 细胞, Plant, 组织, Yeast
运输条件室温
原始材料量Bacteria: ≤107 cells or 0.1 mL Cells: ≤107 Plant: ≤50 mg Tissue: ≤50 mg Yeast: ≤107 cells or 0.1 mL
测试时间30 分钟
分离技术有机提取
Unit SizeEach
内容与储存
内容物:1 瓶 (100 mL) 在 15°C 至 30°C 下储存
常见问题解答 (FAQ)
使用DNAzol试剂分离DNA时,在加入乙醇后出现两相,该如何处理?
如果DNAzol试剂的用量过少,则可能在加入乙醇后出现两相。应加入更多DNAzol试剂并继续实验。
我已经使用DNAzol试剂分离得到了DNA,但使用限制性内切酶无法剪切。原因是什么?
如果在分离DNA前使用磷酸盐缓冲液洗涤细胞或组织,则磷酸盐可能会抑制限制性内切酶。我们推荐向DNA溶液中加入DNAzol试剂,使用0.5倍体积的95%乙醇再次沉淀。沉淀后,使用95%乙醇洗涤2次,短暂干燥,并重悬于8 mM NaOH中。
我使用DNAzol试剂提取得到的DNA很难进行溶解,对此你们有什么建议吗?
您可以尝试将样本溶于8 mM NaOH中,在37°C孵育过夜以帮助DNA的溶解。您也可以尝试在45°C孵育15分钟。
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n/a
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