Isolation and characterization of a cDNA clone encoding human aromatic L-amino acid decarboxylase.
AuthorsIchinose H, Kurosawa Y, Titani K, Fujita K, Nagatsu T
JournalBiochem Biophys Res Commun
PubMed ID2590185
The nucleotide sequence of a cDNA clone that includes the entire coding region of human aromatic L-amino acid decarboxylase gene is presented. A human pheochromocytoma cDNA library was screened using an oligonucleotide probe which corresponded to a partial amino acid sequence of the enzyme purified from the human pheochromocytoma. The ... More
Identification and characterization of a novel form of the human L-dopa decarboxylase mRNA.
L-Dopa decarboxylase (DDC) has been cloned from several species and was shown to undergo alternative splicing within its 5'-untranslated and coding regions. In this report, we describe a novel splice variant of DDC mRNA in human tissue, lacking exons 10-15 of the full-length transcript but including an alternative exon 10. ... More
Reaction and substrate specificity of recombinant pig kidney Dopa decarboxylase under aerobic and anaerobic conditions.
AuthorsBertoldi M, Borri Voltattorni C
JournalBiochim Biophys Acta
PubMed ID12686106
Dopa decarboxylase (DDC) catalyzes as main reaction the stereospecific CO(2) abstraction from L-Dopa and L-5-hydroxytryptophan (5-HTP), generating the corresponding aromatic amines, dopamine and serotonin, respectively. Side reactions with turnover time of minutes are also catalyzed by the enzyme. In particular, DDC exhibits half-transaminase activity toward D-aromatic amino acids and oxidative ... More
Haplotype analysis indicates an association between the DOPA decarboxylase (DDC) gene and nicotine dependence.
DOPA decarboxylase (DDC; also known as L-amino acid decarboxylase; AADC) is involved in the synthesis of dopamine, norepinephrine and serotonin. Because the mesolimbic dopaminergic system is implicated in the reinforcing effects of many drugs, including nicotine, the DDC gene is considered a plausible candidate for involvement in the development of ... More