Membrane topology influences N-glycosylation of the prion protein.
AuthorsWalmsley AR, Zeng F, Hooper NM,
JournalEMBO J
PubMed ID11179215
'The glycosylation state of the glycosyl-phosphatidylinositol (GPI) anchored cellular prion protein (PrPC) can influence the formation of the disease form of the protein responsible for the neurodegenerative spongiform encephalopathies. We have investigated the role of membrane topology in the N-glycosylation of PrP by expressing a C-terminal transmembrane anchored form, PrP-CTM, ... More
The protein SET binds the neuronal Cdk5 activator p35nck5a and modulates Cdk5/p35nck5a activity.
Authors Qu Dianbo; Li Qing; Lim Hui-Ying; Cheung Nam Sang; Li Rong; Wang Jerry H; Qi Robert Z;
JournalJ Biol Chem
PubMed ID11741927
'The neuronal Cdk5 kinase is composed of the catalytic subunit Cdk5 and the activator protein p35(nck5a) or its isoform, p39(nck5ai). To identify novel p35(nck5a)- and p39(nck5ai)-binding proteins, fragments of p35(nck5a) and p39(nck5ai) were utilized in affinity isolation of binding proteins from rat brain homogenates, and the isolated proteins were identified ... More
Production of mice using iPS cells and tetraploid complementation.
AuthorsZhao XY, Lv Z, Li W, Zeng F, Zhou Q,
JournalNat Protoc
PubMed ID20431542
'Induced pluripotent stem cells (iPSCs) are considered to be an attractive alternative to embryonic stem cells (ESCs) and may provide great potential for clinical applications in regenerative medicine. Although possessing characteristics similar to ESCs, the true pluripotency of these newly studied iPSCs was not known because none of the previously ... More