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查看更多产品信息 Rat IgG Isotype Control - FAQs (10700)
4 个常见问题解答
不一定。许多用户使用未染色细胞结合FMO对照，来确定他们的阳性群体。
这些同型对照并不是非特异性结合的最适宜的对照，同型对照的使用正渐渐的离开流式细胞领域。作为替代，可使用未染色细胞确定阴性群体，使用单独染色对照设置补偿，使用减一染色（FMO）对照设置区域和门限。
您可能需要考虑是否有必要使用同型对照。以下是您可能感兴趣的部分参考文献：
•O'Gorman MR, Thomas J. (1999) Isotype controls—time to let go? Cytometry 38:78–80.
•Keeney M, Gratama JW, Chin-Yee IH et al. (1998) Isotype controls in the analysis of lymphocytes and CD34+ stem/progenitor cells by flow cytometry—time to let go! Cytometry 34:280–283.
•Hulspas R, O'Gorman MR, Wood BL et al. (2009) Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry B Clin Cytom 76:355–364.
•Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline—Second Edition. Clinical and Laboratory Standards Institute, (CLSI). Document H42-A2 Volume 27 No.16, 2007.
•Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells; Approved Guideline—Second Edition. Clinical and Laboratory Standards Institute, (CLSI). Document H43-A2 Volume 27 No. 11, 2007.
No. Many users are using unstained cells in combination with FMO controls to identify their positive populations.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The flow cytometry field is moving away from using isotype controls as they are not necessarily the most appropriate way to control for non-specific binding. Instead, they are using unstained cells to define the negative population, single stained controls to set compensation, and flow minus one (FMO) controls to set regions and gates.
You may want to consider whether using an isotype control is something you need to do. Here are some references you might want to look at:
-O'Gorman MR, Thomas J. (1999) Isotype controls-time to let go? Cytometry 38:78-80.
-Keeney M, Gratama JW, Chin-Yee IH et al. (1998) Isotype controls in the analysis of lymphocytes and CD34+ stem/progenitor cells by flow cytometry- time to let go! Cytometry 34:280-283.
-Hulspas R, O'Gorman MR, Wood BL et al. (2009) Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry B Clin Cytom 76:355–364.
-Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline - Second Edition. Clinical and Laboratory Standards Institute, (CLSI). Document H42-A2 Volume 27 No.16, 2007.
-Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells; Approved Guideline - Second Edition. Clinical and Laboratory Standards Institute, (CLSI). Document H43-A2 Volume 27 No. 11, 2007.
If you do wish to use isotype controls, we offer a wide variety of species and fluorophores, search our Web site under "isotype antibody control."
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.