成分确定的角质形成细胞 SFM (1X)
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成分确定的角质形成细胞 SFM (1X)
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成分确定的角质形成细胞 SFM (1X)

成分确定的角质形成细胞 SFM 是一种无血清培养基,经过优化后用于人角质形成细胞的分离和扩增,无需添加牛垂体提取物 (BPE) 或使用成纤维细胞滋养层。BPE 的生长促进活性已被培养基中加入的生长促进剂所取代,从而使产品具有更为一致的性能。这些生长促进剂还可维持细胞形态、生理标志物和生长特性。该培养基的配方可抑制成纤维细胞增殖,其钙浓度较低了解更多信息
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货号数量
10744019500 mL
货号 10744019
价格(CNY)
1,995.00
Each
添加至购物车
数量:
500 mL
价格(CNY)
1,995.00
Each
添加至购物车
成分确定的角质形成细胞 SFM 是一种无血清培养基,经过优化后用于人角质形成细胞的分离和扩增,无需添加牛垂体提取物 (BPE) 或使用成纤维细胞滋养层。BPE 的生长促进活性已被培养基中加入的生长促进剂所取代,从而使产品具有更为一致的性能。这些生长促进剂还可维持细胞形态、生理标志物和生长特性。该培养基的配方可抑制成纤维细胞增殖,其钙浓度较低 (<0.1 mM),因而可用于高浓缩未分化角质化细胞的分离和培养。

成分确定的角质化细胞 SFM 是一种完全无血清培养基,由成分确定的角质化细胞无血清基础培养基组成,并添加成分确定的角质化细胞 SFM 生长添加剂,后者消除了对牛垂体提取物的需求。添加剂与基础培养基分开包装提供。
仅供科研使用。不可用于诊断程序。
规格
细胞类型角质化细胞、上皮细胞
最大浓度1 X
产品类型试剂盒
数量500 mL
有效期12 个月
分类无血清
培养类型哺乳动物细胞培养
形式液体
血清水平无血清
无菌无菌过滤
灭菌方法无菌过滤
加有添加剂谷氨酰胺, 酚红
Unit SizeEach
内容与储存
• 500 mL 成分确定的角质形成细胞-SFM 基础培养基;在冷藏箱 (2-8°C) 中避光保存。
• 1 mL 成分确定的角质形成细胞-SFM 生长添加剂;在冷冻箱(-5 至 -20°C)中避光保存

完整培养基:在冷藏箱 (2-8°C) 中避光保存。

常见问题解答 (FAQ)

Are the Defined Keratinocyte SFM basal medium and supplement available separately?

The Defined Keratinocyte SFM basal medium and supplement are not available separately. The kit can be ordered with catalog #10744-019.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.