Critical contribution of linker proteins to raf kinase activation.
Authors Anselmo Anthony N; Bumeister Ron; Thomas Jackie M; White Michael A;
JournalJ Biol Chem
PubMed ID11741918
'Genetic analysis of Ras signaling has unveiled the participation of non-enzymatic accessory proteins in signal transmission. These proteins, KSR, CNK, and Sur-8, can interact with multiple core components of the Ras/MAP kinase cascade and may contribute to the structural organization of this cascade. However, the precise biochemical nature of the ... More
Crystal Structure of Imaginal Disc Growth Factor-2. A MEMBER OF A NEW FAMILY OF GROWTH-PROMOTING GLYCOPROTEINS FROM DROSOPHILA MELANOGASTER.
Authors Varela Paloma F; Llera Andrea S; Mariuzza Roy A; Tormo Jose;
JournalJ Biol Chem
PubMed ID11821393
'Imaginal disc growth factor-2 (IDGF-2) is a member of a recently described family of Drosophila melanogaster-soluble polypeptide growth factors that promote cell proliferation in imaginal discs. Although their precise mode of action has not been established, IDGFs cooperate with insulin in stimulating the growth of imaginal disc cells. We report ... More
PRODUCTION OF MOSQUITO DENSONUCLEOSIS VIRUSES BY AEDES ALBOPICTUS C6/36 CELLS ADAPTED TO SUSPENSION CULTURE IN SERUM-FREE PROTEIN-FREE MEDIA.
AuthorsSuchman E, Carlson J,
JournalIn Vitro Cell Dev Biol Anim
PubMed ID15311964
Mosquito densonucleosis viruses (MDVs) have the potential for use as biocontrol agents. To facilitate densovirus production, the Aedes albopictus mosquito cell line C6/36 was adapted to two commercially available serum-free protein-free media (SFPFM), Sf-900 II and Drosophila-SFM. Cells adapted more slowly to growth in Sf-900 II medium, but once adapted, ... More
Analysis of Drosophila 26 S proteasome using RNA interference.
Authors Wójcik Cezary; DeMartino George N;
JournalJ Biol Chem
PubMed ID11739392
We have utilized double-stranded RNA interference (RNAi) to examine the effects of reduced expression of individual subunits of the 26 S proteasome in Drosophila S2 cells. RNAi significantly decreased mRNA and protein levels of targeted subunits of both the core 20 S proteasome and the PA700 regulatory complex. Cells deficient ... More