KnockOut™ Serum Replacement

Gibco KnockOut Serum Replacement (KnockOut SR) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this serum replacement has been trusted by many for almost 20 years. Providing the documents required for regulatory review, KnockOut SR reduces your burden in qualifying reagents when transitioning from research applications to clinical applications.

With KnockOut Serum Replacement, you can:

• Culture feeder-dependent PSCs with confidence
• Rely on the improved performance of a qualified, FBS-free formulation
• Enjoy the peace of mind of having a more stable price and supply compared to FBS

Culture PSCs with confidence

KnockOut SR has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.

Rely on improved performance

KnockOut SR has more a defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing PSCs with KnockOut SR leads to less differentiation and more compact colony morphology compared to FBS.

Enjoy the peace of mind of having stable price and supply

KnockOut SR is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.