Prospero-related homeobox (Prox1) is a corepressor of human liver receptor
homolog-1 and suppresses the transcription of the cholesterol 7-alpha-hydroxylase gene.
AuthorsQin J, Gao DM, Jiang QF, Zhou Q, Kong YY, Wang Y, Xie YH,
JournalMol Endocrinol
PubMed ID15205472
Cholesterol 7-alpha-hydroxylase (CYP7A1) catalyzes a rate-limiting step
in bile acid synthesis in liver, and its gene transcription is under complex regulation by multiple nuclear
receptors in response to bile acids, cholesterol derivatives, and hormones. The liver receptor homolog-1
(LRH-1), a member of the fushi tarazu factor 1 subfamily of nuclear receptors, has emerged ... More
BRCA1 interacts with and is required for paclitaxel-induced activation of mitogen-activated protein kinase kinase kinase 3.
AuthorsGilmore PM, McCabe N, Quinn JE, Kennedy RD, Gorski JJ, Andrews HN, McWilliams S, Carty M, Mullan PB, Duprex WP, Liu ET, Johnston PG, Harkin DP,
JournalCancer Res
PubMed ID15205325
'BRCA1 has been implicated in a number of cellular processes, including transcriptional regulation, DNA damage repair, cell cycle arrest, and apoptosis. We identified mitogen-activated protein kinase (MAPK) kinase kinase 3 (MEKK3), an upstream regulator of the c-Jun NH(2)-terminal kinase/stress-activated protein kinase and p38/MAPK pathways, as a novel BRCA1-interacting protein in ... More
Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation.
AuthorsPraetorius-Ibba M, Rogers TE, Samson R, Kelman Z, Ibba M,
JournalJ Biol Chem
PubMed ID15917221
'Aminoacyl-tRNA synthetase-containing complexes have been identified in different eukaryotes, and their existence has also been suggested in some Archaea. To investigate interactions involving aminoacyl-tRNA synthetases in Archaea, we undertook a yeast two-hybrid screen for interactions between Methanothermobacter thermautotrophicus proteins using prolyl-tRNA synthetase (ProRS) as the bait. Interacting proteins identified included ... More
Association of syncoilin and desmin: linking intermediate filament proteins to the dystrophin-associated protein complex.
Authors Poon Ellen; Howman Emily V; Newey Sarah E; Davies Kay E;
JournalJ Biol Chem
PubMed ID11694502
'We recently identified a novel protein called syncoilin, a putative intermediate filament protein that interacts with alpha-dystrobrevin, a member of the dystrophin-associated protein complex. Syncoilin is found at the neuromuscular junction, sarcolemma, and Z-lines and is thought to be important for muscle fiber integrity. Based on the similar protein structure ... More
Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis.
AuthorsSahlender DA, Roberts RC, Arden SD, Spudich G, Taylor MJ, Luzio JP, Kendrick-Jones J, Buss F,
JournalJ Cell Biol
PubMed ID15837803
'Myosin VI plays a role in the maintenance of Golgi morphology and in exocytosis. In a yeast 2-hybrid screen we identified optineurin as a binding partner for myosin VI at the Golgi complex and confirmed this interaction in a range of protein interaction studies. Both proteins colocalize at the Golgi ... More
ATP binding regulates oligomerization and endosome association of RME-1 family proteins.
AuthorsLee DW, Zhao X, Scarselletta S, Schweinsberg PJ, Eisenberg E, Grant BD, Greene LE,
JournalJ Biol Chem
PubMed ID15710626
'Members of the RME-1/mRme-1/EHD1 protein family have recently been shown to function in the recycling of membrane proteins from recycling endosomes to the plasma membrane. RME-1 family proteins are normally found in close association with recycling endosomes and the vesicles and tubules emanating from these endosomes, consistent with the proposal ... More
LIN-12/Notch trafficking and regulation of DSL ligand activity during vulval induction in Caenorhabditis elegans.
AuthorsShaye DD, Greenwald I,
JournalDevelopment
PubMed ID16236769
'A novel mode of crosstalk between the EGFR-Ras-MAPK and LIN-12/Notch pathways occurs during the patterning of a row of vulval precursor cells (VPCs) in Caenorhabditis elegans: activation of the EGFR-Ras-MAPK pathway in the central VPC promotes endocytosis and degradation of LIN-12 protein. LIN-12 downregulation in the central VPC is a ... More
sel-7, a positive regulator of lin-12 activity, encodes a novel nuclear protein in Caenorhabditis elegans.
AuthorsChen J, Li X, Greenwald I,
JournalGenetics
PubMed ID15020414
'Suppressor genetics in C. elegans has identified key components of the LIN-12/Notch signaling pathway. Here, we describe a genetic and molecular characterization of the suppressor gene sel-7. We show that reducing or eliminating sel-7 activity suppresses the effects of constitutive lin-12 activity, enhances the effects of partially reduced lin-12 activity, ... More
p68RacGAP is a novel GTPase-activating protein that interacts with vascular endothelial zinc finger-1 and modulates endothelial cell capillary formation.
AuthorsAitsebaomo J, Wennerberg K, Der CJ, Zhang C, Kedar V, Moser M, Kingsley-Kallesen ML, Zeng GQ, Patterson C,
JournalJ Biol Chem
PubMed ID14966113
'The endothelium is required for maintenance of vascular integrity and homeostasis during vascular development and in adulthood. However, little is known about the coordinated interplay between transcription factors and signaling molecules that regulate endothelial cell-dependent transcriptional events. Vascular endothelial zinc finger-1 (Vezf1) is a zinc finger-containing transcription factor that is ... More
Activity of hypoxia-inducible factor 2alpha is regulated by association with the NF-kappaB essential modulator.
AuthorsBracken CP, Whitelaw ML, Peet DJ,
JournalJ Biol Chem
PubMed ID15653678
'The hypoxia-inducible factors 1alpha (HIF-1alpha) and 2alpha (HIF-2alpha) are key regulators of the transcriptional response to low oxygen and are closely related in domain architecture, DNA binding, and activation mechanisms. Despite these similarities, targeted disruption of the HIF-alpha genes in mice results in distinctly different phenotypes demonstrating nonredundancy of ... More
Novel mutations in the KCNQ2 gene link epilepsy to a dysfunction of the KCNQ2-calmodulin interaction.
AuthorsRichards MC, Heron SE, Spendlove HE, Scheffer IE, Grinton B, Berkovic SF, Mulley JC, Davy A,
JournalJ Med Genet
PubMed ID14985406
Not available
Role of Unc51.1 and its binding partners in CNS axon outgrowth.
AuthorsTomoda T, Kim JH, Zhan C, Hatten ME,
JournalGenes Dev
PubMed ID15014045
Previous studies showed that the serine/threonine kinase Unc51.1 is one of the earliest genes in neuronal differentiation and is required for granule cell axon formation. To examine the mechanism of Unc51.1 regulation of axon extension, we have identified two direct binding partners. The first, SynGAP, a negative regulator of Ras, ... More
The phosphatidylinositol 3-phosphate phosphatase myotubularin- related protein 6 (MTMR6) is a negative regulator of the Ca2+-activated K+ channel KCa3.1.
AuthorsSrivastava S, Li Z, Lin L, Liu G, Ko K, Coetzee WA, Skolnik EY,
JournalMol Cell Biol
PubMed ID15831468
Myotubularins (MTMs) belong to a large subfamily of phosphatases that dephosphorylate the 3' position of phosphatidylinositol 3-phosphate [PI(3)P] and PI(3,5)P(2). MTM1 is mutated in X-linked myotubular myopathy, and MTMR2 and MTMR13 are mutated in Charcot-Marie-Tooth syndrome. However, little is known about the general mechanism(s) whereby MTMs are regulated or the ... More
The MEK1 scaffolding protein MP1 regulates cell spreading by integrating PAK1 and Rho signals.
AuthorsPullikuth A, McKinnon E, Schaeffer HJ, Catling AD,
JournalMol Cell Biol
PubMed ID15923628
How the extracellular signal-regulated kinase (ERK) cascade regulates diverse cellular functions, including cell proliferation, survival, and motility, in a context-dependent manner remains poorly understood. Compelling evidence indicates that scaffolding molecules function in yeast to channel specific signals through common components to appropriate targets. Although a number of putative ERK scaffolding ... More
Interaction of hREV1 with three human Y-family DNA polymerases.
AuthorsOhashi E, Murakumo Y, Kanjo N, Akagi J, Masutani C, Hanaoka F, Ohmori H,
JournalGenes Cells
PubMed ID15189446
Polkappa is one of many DNA polymerases involved in translesion DNA synthesis (TLS). It belongs to the Y-family of polymerases along with Poleta, Poliota and hREV1. Unlike Poleta encoded by the xeroderma pigmentosum variant (XPV) gene, Polkappa is unable to bypass UV-induced DNA damage in vitro, but it is able ... More
Composition and architecture of the Schizosaccharomyces pombe Rad18 (Smc5-6) complex.
AuthorsSergeant J, Taylor E, Palecek J, Fousteri M, Andrews EA, Sweeney S, Shinagawa H, Watts FZ, Lehmann AR,
JournalMol Cell Biol
PubMed ID15601840
The rad18 gene of Schizosaccharomyces pombe is an essential gene that is involved in several different DNA repair processes. Rad18 (Smc6) is a member of the structural maintenance of chromosomes (SMC) family and, together with its SMC partner Spr18 (Smc5), forms the core of a high-molecular-weight complex. We show here ... More
A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome.
Authors Davy A; Bello P; Thierry-Mieg N; Vaglio P; Hitti J; Doucette-Stamm L; Thierry-Mieg D; Reboul J; Boulton S; Walhout A J; Coux O; Vidal M;
JournalEMBO Rep
PubMed ID11559592
The ubiquitin-proteasome proteolytic pathway is pivotal in most biological processes. Despite a great level of information available for the eukaryotic 26S proteasome-the protease responsible for the degradation of ubiquitylated proteins-several structural and functional questions remain unanswered. To gain more insight into the assembly and function of the metazoan 26S proteasome, ... More