LB Broth (1X), 1 L - FAQs

进行转化时我需要额外准备哪些材料？

您将需要细菌生长培养基，例如LB，细菌筛选用的抗生素，以及诱导剂（IPTG, X-gal）。我们提供用于培养E. Coli菌株的imMedia生长培养基，它是一种预混的、预先灭菌的培养基，包含筛选用抗生素。制备过程中可以加入或者不加入IPTG和X-gal。

我应该使用哪种细菌生长培养基？

请查看我们的细菌生长培养基列表（https://www.thermofisher.com/cn/zh/home/life-science/cell-culture/microbiological-culture/bacterial-growth-media.html）。

What additional materials do I need for my transformation?

You will need bacterial growth media, such as LB, the bacterial selection antibiotic, and induction reagent (IPTG, X-gal). We offer imMedia growth medium which is a pre-mixed, pre-sterilized medium containing selection antibiotics for growth of E. coli strains. It can be prepared with or without IPTG and X-gal.

What bacterial growth media should I use?

Check out our bacterial growth media chart (https://www.thermofisher.com/us/en/home/life-science/cell-culture/microbiological-culture/bacterial-growth-media.html).

What is the optimal pH of low salt LB for LB + blasticidin plates?

We recommend a pH of 7 or less and half the normal amount of NaCl in your LB media or plates.

See the following paper for details on optimal conditions: Yamaguchi et al (1965) Inhibition of Protein Synthesis by Blasticidin S. Journal of Biochemistry (Tokyo) Volume 57: pp 667-677.