Enhancing yield of infectious Bursal disease virus structural proteins in baculovirus expression systems: focus on media, protease inhibitors, and dissolved oxygen.
Authors Hu Y C; Bentley W E;
JournalBiotechnol Prog
PubMed ID10585191
'Structural proteins of the poultry pathogen, infectious bursal disease virus (IBDV), were expressed in the baculovirus/insect cell expression system. To date, several reports have indicated that animal virus structural proteins are expressed only at low yield in this system. In this article, several factors were examined to enhance yield. These ... More
When less is more: enhanced baculovirus production of recombinant proteins at very low multiplicities of infection.
Authors Liebman J M; LaSala D; Wang W; Steed P M;
JournalBiotechniques
PubMed ID9894589
Enhanced growth of Sf-9 cells to a maximum density of 5.2 x 10(7) cells per mL and production of beta-galactosidase at high cell density by fed batch culture.