Enhancing yield of infectious Bursal disease virus structural proteins in baculovirus expression systems: focus on media, protease inhibitors, and dissolved oxygen.
Authors Hu Y C; Bentley W E;
JournalBiotechnol Prog
PubMed ID10585191
'Structural proteins of the poultry pathogen, infectious bursal disease virus (IBDV), were expressed in the baculovirus/insect cell expression system. To date, several reports have indicated that animal virus structural proteins are expressed only at low yield in this system. In this article, several factors were examined to enhance yield. These ... More
Titration of non-occluded baculovirus using a cell viability assay.
AuthorsMena JA, RamÃrez OT, Palomares LA,
JournalBiotechniques
PubMed ID12613247
Enhanced growth of Sf-9 cells to a maximum density of 5.2 x 10(7) cells per mL and production of beta-galactosidase at high cell density by fed batch culture.
Authors Arosio Daniele; Cui Sheng; Ortega Claudia; Chovanec Miroslav; Di Marco Stefania; Baldini Giancarlo; Falaschi Arturo; Vindigni Alessandro;
JournalJ Biol Chem
PubMed ID11796732
The Ku heterodimer plays a central role in non-homologous end-joining. The binding of recombinant Ku to DNA has been investigated by dynamic light scattering, double-filter binding, fluorescence spectroscopy, and band shift assays. The hydrodynamic radius of Ku in solution is 5.2 nm and does not change when a 25-bp double-strand ... More