Sf-900™ II SFM, 6 x 1 L - FAQs

View additional product information for Sf-900™ II SFM - FAQs (10902088, 10902096, 10902104)

7 product FAQs found

昆虫细胞培养可使用哪些种类和浓度的杀菌剂？

许多抗生素都适用于昆虫细胞。下列为常用抗生素：

青霉素/链霉素：50–100 U/mL；50–100 μg/mL
两性霉素B(Fungizone 抗真菌剂）：0.25 μg/mL
庆大霉素：500 mL培养基中加入0.5 mL 10 mg/mL溶液（最终浓度10 μg/mL）

在将血清加入我的培养基之前，是否需要对其进行热灭活操作？

热灭活操作不是必需的。我们的团队直接使用未经热灭活的血清，并未发现其对细胞生长或细胞形态有任何显著影响。

你们是否提供无血清的昆虫培养基？

是的，我们提供各类无血清的昆虫培养基，请点击此处(https://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2)浏览我们所提供的培养基以及它们之间的区别。

Can Sf9 cells in Sf-900 III SFM (Cat. No. 12659017) be thawed and grown in Sf-900 II SFM instead?

It should be okay to thaw the cells into Sf-900 II SFM. This is a richer media compared to the Sf-900 III SFM so the cells would have an easy time adapting. We would recommend taking the cells through 3 passages in the new medium before using them for any experiments as that they have enough time to adapt.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antimicrobials can be used in insect culture and at what concentration?

Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to heat-inactivate my serum before adding it to my medium?

Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.

Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer serum-free insect media?

Yes, we offer a variety of serum-free insect media. Please go here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2) to view the media we offer and the differences between them.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.