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View additional product information for Sf-900™ II SFM - FAQs (10902187, 10902179, 10902161)
17 product FAQs found
许多抗生素都适用于昆虫细胞。下列为常用抗生素：
青霉素/链霉素：50–100 U/mL；50–100 μg/mL
两性霉素B(Fungizone 抗真菌剂）：0.25 μg/mL
庆大霉素：500 mL培养基中加入0.5 mL 10 mg/mL溶液（最终浓度10 μg/mL）
热灭活操作不是必需的。我们的团队直接使用未经热灭活的血清，并未发现其对细胞生长或细胞形态有任何显著影响。
是的，我们提供各类无血清的昆虫培养基，请点击此处(https://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2)浏览我们所提供的培养基以及它们之间的区别。
有的。尽管我们感觉PE膜材料在技术性能上超越了市面上许多其他膜材料，我们仍将以订购的形式继续供应和支持我们目前正在销售的其他膜材料。
我们对每一尺寸的培养袋都设计了特定重量的瓦楞纸箱来辅助运输：
5 L: 12” x 14.25” x 5.5”
10 L: 13” x 23.5” x 6.125”
20 L: 15” x 24” x 6.125”
这一市销的膜材料是一种非常透明，多层的单卷膜材料，并拥有聚乙烯接触表面，可制成各种不同的尺寸（250 mL至1000 L）。此外，这一膜材料还具有卓越的气体阻隔性能和极低渗漏率，是市场的领先产品。点击此处 了解更多关于PE膜材料的说明。
基于用户的反馈，我们设计了一个袋装培养基的系列，所有尺寸的培养袋均使用一致的膜体进行包装。这一膜材料——称为PE膜——比其他市售膜材料的功能更优。点击此处了解更多关于PE膜材料的说明。
Gibco细胞培养袋（Cell Culture Bag）是预先灌注培养基，使用方便的包装，具有多种配方与体积，特别针对提升效能与降低成本的需求进行了专门设计。这些培养袋的体积可达1000升。请点击此处阅读更多关于这些培养袋的相关信息。
It should be okay to thaw the cells into Sf-900 II SFM. This is a richer media compared to the Sf-900 III SFM so the cells would have an easy time adapting. We would recommend taking the cells through 3 passages in the new medium before using them for any experiments as that they have enough time to adapt.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.
Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, we offer a variety of serum-free insect media. Please go here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2) to view the media we offer and the differences between them.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes. Although we feel that the PE film exceeds many of the technical properties of the other available films, we will continue to offer and support our other currently available films through custom orders.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Each bag size will arrive in a specific heavyweight corrugated box designed for shipment:
5 L: 12” x 14.25” x 5.5”
10 L: 13” x 23.5” x 6.125”
20 L: 15” x 24” x 6.125”
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The commercially available film is a very clear, multi-layered, single web film with a polyethylene contact surface that can be made into a wide range of bag sizes (250 mL to 1,000L). In addition, the film is a market leader with exceptional gas barrier properties and very low extractable and leachable results. Click here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Based on feedback from customers, we designed a media bag portfolio that uses a consistent film for all bag sizes. This film, called PE film, is superior in function to other commercially available films. Please go here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Gibco Cell Culture Bags are convenient, pre-filled bags in a variety of formulations and sizes, specifically designed to increase productivity and reduce costs. The bags can be formatted up to 1,000 L. Read more about these bags here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.