Molecular and functional analysis of the lepB gene, encoding a type I signal peptidase from Rickettsia rickettsii and Rickettsia typhi.
AuthorsRahman MS, Simser JA, Macaluso KR, Azad AF,
JournalJ Bacteriol
PubMed ID12867468
'The type I signal peptidase lepB genes from Rickettsia rickettsii and Rickettsia typhi, the etiologic agents of Rocky Mountain spotted fever and murine typhus, respectively, were cloned and characterized. Sequence analysis of the cloned lepB genes from R. rickettsii and R. typhi shows open reading frames of 801 and 795 ... More
Acetylcholine-induced phosphatidylinositol 4,5-bisphosphate depletion does not cause short-term desensitization of G protein-gated inwardly rectifying K+ current in mouse atrial myocytes.
Authors Cho Hana; Hwang Ji-Young; Kim Daesoo; Shin Hee-Sup; Kim Yangmi; Earm Yung E; Ho Won-Kyung;
JournalJ Biol Chem
PubMed ID12019267
'Depletion of phosphatidylinositol 4,5-bisphosphate (PIP(2)) induced by phenylephrine or endothelin causes the inhibition of acetylcholine-activated K(+) current (I(KACh)) in atrial myocytes. In the present study, we have investigated the hypothesis that muscarinic receptor induced PIP(2) depletion also causes inhibition of I(KACh), resulting in desensitization. We confirmed the expression of G(q)-coupled ... More
Phosphorylation regulates the subcellular location and activity of the snail transcriptional repressor.
AuthorsDomÃnguez D, Montserrat-SentÃs B, Virgós-Soler A, Guaita S, Grueso J, Porta M, Puig I, Baulida J, Francà C, GarcÃa de Herreros A,
JournalMol Cell Biol
PubMed ID12832491
'The Snail gene product is a transcriptional repressor of E-cadherin expression and an inducer of the epithelial-to-mesenchymal transition in several epithelial tumor cell lines. This report presents data indicating that Snail function is controlled by its intracellular location. The cytosolic distribution of Snail depended on export from the nucleus by ... More
Calcium regulation of matrix metalloproteinase-mediated migration in oral squamous cell carcinoma cells.
AuthorsMunshi HG, Wu YI, Ariztia EV, Stack MS,
JournalJ Biol Chem
PubMed ID12194986
'Activation of matrix metalloproteinase 2 (MMP-2) has been shown to play a significant role in the behavior of cancer cells, affecting both migration and invasion. The activation process requires multimolecular complex formation involving pro-MMP-2, membrane type 1-MMP (MT1-MMP), and tissue inhibitor of metalloproteinases-2 (TIMP-2). Because calcium is an important regulator ... More
Endothelin-1 Induces Vascular Endothelial Growth Factor by Increasing Hypoxia-inducible Factor-1alpha in Ovarian Carcinoma Cells.
'Angiogenesis is an essential prerequisite for tumor growth, invasion, and metastasis. In ovarian carcinoma cells, endothelin-1 (ET-1) stimulates the secretion of vascular endothelial growth factor (VEGF), a major mediator of tumor angiogenesis. In OVCA 433 and HEY ovarian carcinoma cell lines, ET-1 treatment increases VEGF mRNA expression and induces VEGF ... More
A single point mutation in ecdysone receptor leads to increased ligand specificity: Implications for gene switch applications.
Authors Kumar M B; Fujimoto T; Potter D W; Deng Q; Palli S R;
JournalProc Natl Acad Sci U S A
PubMed ID12411578
'The ecdysone receptor (EcR), a member of the nuclear receptor superfamily, plays an important role in regulating development and reproduction in insects. The EcR binds to ecdysteroids and regulates transcription of genes that contain ecdysone response elements. The EcR has been used to develop inducible gene switches for efficient regulation ... More
Broad requirement for the mediator subunit RGR-1 for transcription in the Caenorhabditis elegans embryo.
AuthorsShim EY, Walker AK, Blackwell TK
JournalJ Biol Chem
PubMed ID12089139
'The Mediator-related transcription co-factors integrate positive and negative inputs and recruit and activate the RNA polymerase II complex. To understand the role of Mediator during transcription, it is important to identify Mediator subunits that are essential for its functions. In the yeast Mediator, the conserved component Rgr1 is associated with ... More
Transcriptional Regulation of the Pituitary Vasopressin V1b Receptor Involves a GAGA-binding Protein.
'The role of CT repeats (inverted GAGA box) in the rat vasopressin V1b receptor (V1bR) promoter in the transcriptional regulation of this gene was studied in H32 hypothalamic cells, which express endogenous V1bR. Transfection of a 2.5-kb V1bR fragment (2161 bp upstream and 377 bp downstream of the proximal transcriptional ... More
Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR.
'Viral hemorrhagic fevers (VHFs) are acute infections with high case fatality rates. Important VHF agents are Ebola and Marburg viruses (MBGV/EBOV), Lassa virus (LASV), Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), dengue virus (DENV), and yellow fever virus (YFV). VHFs are clinically difficult to diagnose and to ... More
Maintenance of Integrated Proviral Gene Expression Requires Brm, a Catalytic Subunit of SWI/SNF Complex.
Authors Mizutani Taketoshi; Ito Taiji; Nishina Mitsue; Yamamichi Nobutake; Watanabe Akiko; Iba Hideo;
JournalJ Biol Chem
PubMed ID11850427
'We show here that murine leukemia virus-based retrovirus vector transgene expression is rapidly silenced in human tumor cell lines lacking expression of Brm, a catalytic subunit of the SWI/SNF chromatin remodeling complex, even though these vectors can successfully enter, integrate, and initiate transcription. We detected this gene silencing as a ... More
BRCA1 supports XIST RNA concentration on the inactive X chromosome.
Authors Ganesan Shridar; Silver Daniel P; Greenberg Roger A; Avni Dror; Drapkin Ronny; Miron Alexander; Mok Samuel C; Randrianarison Voahangy; Brodie Steven; Salstrom Jennifer; Rasmussen Theodore P; Klimke Ann; Marrese Christine; Marahrens York; Deng Chu Xia; Feunteun Jean; Livingston David M;
JournalCell
PubMed ID12419249
BRCA1, a breast and ovarian tumor suppressor, colocalizes with markers of the inactive X chromosome (Xi) on Xi in female somatic cells and associates with XIST RNA, as detected by chromatin immunoprecipitation. Breast and ovarian carcinoma cells lacking BRCA1 show evidence of defects in Xi chromatin structure. Reconstitution of BRCA1-deficient ... More
A potential role for RNA interference in controlling the activity of the human LINE-1 retrotransposon.
AuthorsSoifer HS, Zaragoza A, Peyvan M, Behlke MA, Rossi JJ,
JournalNucleic Acids Res
PubMed ID15701756
Long interspersed nuclear elements (LINE-1 or L1) comprise 17% of the human genome, although only 80-100 L1s are considered retrotransposition-competent (RC-L1). Despite their small number, RC-L1s are still potential hazards to genome integrity through insertional mutagenesis, unequal recombination and chromosome rearrangements. In this study, we provide several lines of evidence ... More
Novel GC-rich DNA-binding compound produced by a genetically engineered mutant of the mithramycin producer Streptomyces argillaceus exhibits improved transcriptional repressor activity: implications for cancer therapy.
AuthorsAlbertini V, Jain A, Vignati S, Napoli S, Rinaldi A, Kwee I, Nur-e-Alam M, Bergant J, Bertoni F, Carbone GM, Rohr J, Catapano CV,
JournalNucleic Acids Res
PubMed ID16571899
The aureolic acid antibiotic mithramycin (MTM) binds selectively to GC-rich DNA sequences and blocks preferentially binding of proteins, like Sp1 transcription factors, to GC-rich elements in gene promoters. Genetic approaches can be applied to alter the MTM biosynthetic pathway in the producing microorganism and obtain new products with improved pharmacological ... More
Activation of the JNK pathway during dorsal closure in Drosophila requires the mixed lineage kinase, slipper.
Authors Stronach Beth; Perrimon Norbert;
JournalGenes Dev
PubMed ID11825878
The Jun kinase (JNK) pathway has been characterized for its role in stimulating AP-1 activity and for modulating the balance between cell growth and death during development, inflammation, and cancer. Six families of mammalian kinases acting at the level of JNKKK have emerged as upstream regulators of JNK activity (MLK, ... More
An endocrine pathway in the prostate, ERbeta, AR, 5alpha-androstane-3beta,17beta-diol, and CYP7B1, regulates prostate growth.
Authors Weihua Zhang; Lathe Richard; Warner Margaret; Gustafsson Jan-Ake;
JournalProc Natl Acad Sci U S A
PubMed ID12370428
Epithelial proliferation of the ventral prostate in rodents peaks between 2 and 4 weeks of age, and by week 8, proliferating cells are rare. We have used ERbeta(-/-) and CYP7B1(-/-) mice to investigate the role of ERbeta and one of its ligands, 5alpha-androstane-3beta,17beta-diol (3betaAdiol), in growth of the ventral prostate. ... More
Snail induction of epithelial to mesenchymal transition in tumor cells is accompanied by MUC1 repression and ZEB1 expression.
AuthorsGuaita S, Puig I, Franci C, Garrido M, Dominguez D, Batlle E, Sancho E, Dedhar S, De Herreros AG, Baulida J
JournalJ Biol Chem
PubMed ID12161443
E-cadherin protein plays a key role in the establishment and maintenance of adherent junctions. Recent evidence implicates the transcription factor Snail in the blockage of E-cadherin expression in fibroblasts and some epithelial tumor cells through direct binding to three E-boxes in the E-cadherin promoter. Transfection of Snail into epithelial cells ... More
p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas.
Interactions between the p53 and PI3K/AKT pathways play a significant role in the determination of cell death/survival. In benign cells these pathways are interrelated through the transcriptional regulation of PTEN by p53, which is required for p53-mediated apoptosis. PTEN exerts its effects by decreasing the phosphorylated AKT fraction, thereby diminishing ... More
Distinct recognition by two subsets of T cells of an MHC class II-peptide complex.
Authors Pu Zheng; Carrero Javier A; Unanue Emil R;
JournalProc Natl Acad Sci U S A
PubMed ID12084929
We examine here the nature of the differential recognition by CD4+ T cells of a single peptide from hen-egg white lysozyme (HEL) presented by I-A(k) class II MHC molecules. Two subsets of T cells (called A and B) interact with the same peptide, each in unique ways that reflect the ... More
Haploinsufficiency of the Mouse Forkhead Box f1 Gene Causes Defects in Gall Bladder Development.
Authors Kalinichenko Vladimir V; Zhou Yan; Bhattacharyya Dibyendu; Kim Wooram; Shin Brian; Bambal Kalyani; Costa Robert H;
JournalJ Biol Chem
PubMed ID11809759
The forkhead box f1 (Foxf1) transcription factor is expressed in the visceral (splanchnic) mesoderm, which is involved in mesenchymal-epithelial signaling required for development of organs derived from foregut endoderm such as lung, liver, gall bladder, and pancreas. Our previous studies demonstrated that haploinsufficiency of the Foxf1 gene caused pulmonary abnormalities ... More
The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases.
Stem rust caused by Puccinia graminis f. sp. tritici was among the most devastating diseases of barley in the northern Great Plains of the U.S. and Canada before the deployment of the stem rust-resistance gene Rpg1 in 1942. Since then, Rpg1 has provided durable protection against stem rust losses in ... More
Cyclooxygenase-2 and presenilin-1 gene expression induced by interleukin-1beta and amyloid beta 42 peptide is potentiated by hypoxia in primary human neural cells.
AuthorsBazan NG, Lukiw WJ.
JournalJ Biol Chem
PubMed ID12050157
Lipid messengers and amyloid beta (Abeta) peptides generated by cyclooxygenase-2 (COX-2) and presenilin-1 (PS1) mediate pro-inflammatory signaling and neural degeneration in Alzheimer's disease (AD) brain. This study provides data showing that the COX-2 and PS1 genes each transcribe rare, highly labile RNA species that display early response gene behavior in ... More
Identification of genes downstream of Pax6 in the mouse lens using cDNA microarrays.
Pax6 is a transcription factor that regulates the development of the visual, olfactory, and central nervous systems, pituitary, and pancreas. Pax6 is required for induction, growth, and maintenance of the lens; however, few direct Pax6 target genes are known. This study was designed to identify batteries of differentially expressed genes ... More
E2F mediates cell cycle-dependent transcriptional repression in vivo by recruitment of an HDAC1/mSin3B corepressor complex.
Authors Rayman Joseph B; Takahashi Yasuhiko; Indjeian Vahan B; Dannenberg Jan-Hermen; Catchpole Steven; Watson Roger J; te Riele Hein; Dynlacht Brian David;
JournalGenes Dev
PubMed ID11959842
Despite biochemical and genetic data suggesting that E2F and pRB (pocket protein) families regulate transcription via chromatin-modifying factors, the precise mechanisms underlying gene regulation by these protein families have not yet been defined in a physiological setting. In this study, we have investigated promoter occupancy in wild-type and pocket protein-deficient ... More
The p53MH algorithm and its application in detecting p53-responsive genes.
Authors Hoh J; Jin S; Parrado T; Edington J; Levine A J; Ott J;
JournalProc Natl Acad Sci U S A
PubMed ID12077306
A computer algorithm, p53MH, was developed, which identifies putative p53 transcription factor DNA-binding sites on a genomewide scale with high power and versatility. With the sequences from the human and mouse genomes, putative p53 DNA-binding elements were identified in a scan of 2,583 human genes and 1,713 mouse orthologs based ... More
Cathepsin L is essential for embryogenesis and development of Caenorhabditis elegans.
Authors Hashmi Sarwar; Britton Collette; Liu Jing; Guiliano David B; Oksov Yelena; Lustigman Sara;
JournalJ Biol Chem
PubMed ID11707440
Cysteine proteases play critical biological roles in both intracellular and extracellular processes. We characterized Ce-cpl-1, a Caenorhabditis elegans cathepsin L-like cysteine protease. RNA interference with Ce-cpl-1 activity resulted in embryonic lethality and a transient delayed growth of larvae to egg producing adults, suggesting an essential role for cpl-1 during embryogenesis, ... More