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Citations & References (17)
Invitrogen™
Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
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| Catalog Number | No. of Reactions |
|---|---|
| 10966018 | 120 Reactions |
| 10966026 | 300 Reactions |
| 10966034 | 600 Reactions |
| 10966083 | 5000 Reactions |
Catalog number 10966018
Price (CNY)
1,139.00
Each
No. of Reactions:
120 Reactions
Price (CNY)
1,139.00
Each
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.
The hot-start property allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.
Features
- Convenient reaction assembly at room temperature
- 5' to 3' exonuclease activity
- Lacks 3' to 5' exonuclease activity
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- Hot-start PCR
- RT-PCR
Notes
- For superior PCR performance, the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase is recommended.
- Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatTube
Hot StartBuilt-In Hot Start
No. of Reactions120 Reactions
Overhang3'-A
PolymerasePlatinum Taq DNA Polymerase
Product TypeDNA Polymerase
Quantity120 reactions
Reaction FormatSeparate Components
Shipping ConditionWet or Dry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceLow
Reaction SpeedFast or Standard
Unit SizeEach
Contents & Storage
• Platinum Taq DNA Polymerase, 24 μL
• 10X PCR Buffer (without magnesium), 1.25 mL
• 50 mM MgCl2, 1 mL
• KB Extender, 1.25 mL
Store at -10°C to -30°C in a non-frost-free freezer.
• 10X PCR Buffer (without magnesium), 1.25 mL
• 50 mM MgCl2, 1 mL
• KB Extender, 1.25 mL
Store at -10°C to -30°C in a non-frost-free freezer.
Frequently asked questions (FAQs)
Can I purchase just the 10X PCR buffer that comes with Platinum Taq?
My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?
The primers I am using worked for PCR initially, but over time, have stopped working. What happened?
I don't see a pellet in my oligo tube order. Should I ask for a replacement?
There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?
Citations & References (17)
Citations & References
Abstract
De novo ceramide regulates the alternative splicing of caspase 9 and Bcl-x in A549 lung adenocarcinoma cells. Dependence on protein phosphatase-1.
Journal:J Biol Chem
PubMed ID:11801602
'In a recent study, we showed that ceramide induces the dephosphorylation of SR proteins, a family of protein factors that regulate alternative splicing. In this study, the regulation of the alternative processing of pre-mRNA of both caspase 9 and Bcl-x(L) was examined in response to ceramide. Treatment of A549 lung
Quantitative Assessment of Gene Targeting in Vitro and in Vivo by the Pancreatic Transcription Factor, Pdx1. IMPORTANCE OF CHROMATIN STRUCTURE IN DIRECTING PROMOTER BINDING.
Journal:J Biol Chem
PubMed ID:11825903
'The transcription factor Pdx1 is expressed in the pancreatic beta-cell, where it is believed to regulate several beta-cell-specific genes. Whereas binding by Pdx1 to elements of beta-cell genes has been demonstrated in vitro, almost none of these genes has been demonstrated to be a direct binding target for Pdx1 within
Angiotensin II inhibits human trophoblast invasion through AT1 receptor activation.
Journal:J Biol Chem
PubMed ID:11983698
Trophoblast implantation depends, in part, on the controlled production of plasmin from plasminogen, a process regulated by plasminogen activators and plasminogen activator inhibitors. We have determined that angiotensin II (Ang II) stimulates plasminogen activator inhibitor-1 (PAI-1) synthesis and secretion in human trophoblasts in a time- and concentration-dependent manner. Our results
Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin.
Journal:Cell
PubMed ID:12086669
Nerves and blood vessels are branched structures, but whether their branching patterns are established independently or coordinately is not clear. Here we show that arteries, but not veins, are specifically aligned with peripheral nerves in embryonic mouse limb skin. Mutations that eliminate peripheral sensory nerves or Schwann cells prevent proper
Translational regulation of BACE-1 expression in neuronal and non-neuronal cells.
Journal:Nucleic Acids Res
PubMed ID:15034149
As the main beta-secretase of the central nervous system, BACE-1 is a key protein in the pathogenesis of Alzheimer's disease. Excessive expression of the protein might cause an overproduction of the neurotoxic beta-amyloid peptide. Therefore, a tight regulation of BACE-1 expression is expected in vivo. In addition to a possible