Sf-900™ Medium (1.3X) - FAQs

Q: 为了开展噬斑测定实验，你们推荐使用何种培养基来培育Sf9或Sf21细胞？

我们推荐您使用我们的Graces supplemented昆虫培养基（货号11667037）或SF-900培养基（1.3X）（货号10967032）。 Graces Supplemented昆虫培养基是2X的配方培养基，专为在噬斑测定实验琼脂糖覆盖层中的应用而配制。本培养基经以下成份的微调： 乳清蛋白水解物 酵母粉 L-谷氨酰胺 碳酸氢钠 点击此处(https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html)获取完整配方。 SF-900培养基（1.3X）是为Sf9和Sf21细胞准备的不含血清的低蛋白昆虫细胞培养基，适用于在噬斑测定实验1%的琼脂糖覆盖层中使用。

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2 个常见问题解答

为了开展噬斑测定实验，你们推荐使用何种培养基来培育Sf9或Sf21细胞？

我们推荐您使用我们的Grace’s supplemented昆虫培养基（货号11667037）或SF-900培养基（1.3X）（货号10967032）。
Grace’s Supplemented昆虫培养基是2X的配方培养基，专为在噬斑测定实验琼脂糖覆盖层中的应用而配制。本培养基经以下成份的微调：
乳清蛋白水解物
酵母粉
L-谷氨酰胺
碳酸氢钠
点击此处(https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html)获取完整配方。

SF-900培养基（1.3X）是为Sf9和Sf21细胞准备的不含血清的低蛋白昆虫细胞培养基，适用于在噬斑测定实验1%的琼脂糖覆盖层中使用。

What medium do you recommend to grow Sf9 or Sf21 cells for performing plaque assays?

We would recommend using our Grace's Supplemented Insect Medium (Cat. No. 11667037) or SF-900 Medium (1.3X) (Cat. No. 10967032). Grace's Supplmented Insect Media is a 2X formulation intended for use in the agarose overlay when performing the plaque assay. The media has been modified with:
- Lactalbumin hydrolysate
- Yeastolate
- L-glutamine
- Sodium bicarbonate

Please go here for the complete formulation (http://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html). SF-900 Medium (1.3X) is a complete serum-free low protein insect cell culture medium for Sf9 and Sf21 cells, which can be used for 1% agarose overlays used for plaque assays.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.