What medium do you recommend to grow Sf9 or Sf21 cells for performing plaque assays?
We would recommend using our Grace's Supplemented Insect Medium (Cat. No. 11667037) or SF-900 Medium (1.3X) (Cat. No. 10967032).
Grace's Supplmented Insect Media is a 2X formulation intended for use in the agarose overlay when performing the plaque assay. The media has been modified with:
- Lactalbumin hydrolysate
- Yeastolate
- L-glutamine
- Sodium bicarbonate
Please go here for the complete formulation (http://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html). SF-900 Medium (1.3X) is a complete serum-free low protein insect cell culture medium for Sf9 and Sf21 cells, which can be used for 1% agarose overlays used for plaque assays.