Plant DNAzol™ Reagent - FAQs

View additional product information for Plant DNAzol™ Reagent - FAQs (10978021)

7 product FAQs found

DNAzol试剂只能用来分离基因组DNA吗？还是也可用于分离质粒DNA？能用于分离线粒体DNA吗？

使用DNAzol试剂分离得到的实际上是总DNA，所以质粒DNA也会和基因组DNA一并分离出来。线粒体基因组与质粒类似，也可通过DNAzol试剂分离出来。可将离心步骤之前的乙醇室温孵育步骤从1分钟延长至5-10分钟，以获得最大的DNA得率。

DNAzol试剂应储存于何种温度下才可保持其有效性？实验方案中有无可以暂停/暂存的时间点？

我们推荐将DNAzol试剂储存于室温。DNAzol的裂解物（匀浆）可于15–30°C下储存1个月；在4°C或–20°C条件下储存10个月之后，DNAzol裂解物（匀浆）中仍能够提取得到高分子量的、可被限制性内切酶完全酶切的基因组DNA，且DNA在PCR反应中也可以得到较好的结果。在漂洗阶段，DNA可以保存于95%的乙醇中，在15°C至30°C至少储存一周，或4°C左右条件下至少储存三个月。DNA保存于DNAzol试剂中，可于室温下储存一个月，或4°C下储存十个月。

我想分离植物DNA，但有点担心组织中碳水化合物和复杂多糖的含量过高。你们推荐使用哪种试剂盒？

根据起始样品量和纯化方式的不同，我们专门设计了几种针对植物DNA提取的试剂盒。请点击此 链接（https://www.thermofisher.com/it/en/home/life-science/dna-rna-purification-analysis/genomic-dna-extraction/genomic-dna-extraction-sample-type.html?open=plants-accordion）了解我们目前供应的各种植物DNA提取试剂盒的特点。

Will DNAzol Reagent isolate only genomic DNA or will plasmid DNA also be isolated? How about mitochondrial DNA?

The DNA isolated is actually total DNA, so plasmid DNA will be isolated along with genomic DNA. The mitochondrial genome is similar to a plasmid and can be isolated using DNAzol Reagent. The 1 minute room temperature incubation in ethanol before centrifugation should be extended to 5-10 minutes for maximum recovery.

At what temperatures can DNAzol reagent be stored and still be okay to use? Are there possible stopping/storage points in the protocol?

We recommend storing DNAzol Reagent at room temperature. The DNAzol lysate (homogenate) can be stored 1 month at 15-30 degrees C; after 10 months at 4 degrees C or -20 degrees C, the DNAzol lysate (homogenate) has yielded high molecular weight genomic DNA, which can be completely digested with restriction enzymes and works well in PCR. During washes, DNA can be stored in 95% EtOH for at least one week at 15 degrees C to 30 degrees C or for three months at approximately 4 degrees C. DNA can be stored in DNAzol Reagent for one month at room temperature or 10 months at 4 degrees C.

I'm interested in isolating plant DNA, but am worried about the abundance of carbohydrates and complex polysaccharides in the tissue. What kit do you recommend for this?

We offer several kits specifically designed to isolate plant DNA depending on the amount of starting material and chemistry you are interested in using. Please use this link for a comparison of our kits available for plant DNA extraction.

After DNAzol reagent extraction, my OD 260/280 ratio is low. Does this mean I have poor-quality DNA?

Consider the following if you have a low 260/280 ratio:

The correct amount of DNAzol reagent may not have been used. If DNAzol reagent was added to a cell pellet, make sure that the volume of reagent is 20 times that of the cell pellet.

There may have been a problem in pipetting away the viscous supernatant from the DNA pellet, leading to contamination with protein. The DNA may be used with DNAzol reagent again or extracted with phenol to remove the protein.

In some samples dissolved in water, the ratio may be low due to the acidity of the water or the low ion content in the water. The ratios may go up if the sample is dissolved in TE and the spec is zeroed with TE (or 1 to 3 mM Na2PO4, pH ~8.0). [See BioTechniques 22:474-6, 478-81 (1997).]. The molar extinction coefficient of the nucleotides is given at neutral pH, suggesting that the absorbance at 260 nm would be highest at neutral pH. Of course, DNA is not stable under acidic conditions so degradation may occur if the DNA is left in this condition for too long.