EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily.
AuthorsStipp CS, Kolesnikova TV, Hemler ME
JournalJ Biol Chem
PubMed ID11504738
'A novel Ig superfamily protein, EWI-2, was co-purified with tetraspanin protein CD81 under relatively stringent Brij 96 detergent conditions and identified by mass spectrometric protein sequencing. EWI-2 associated specifically with CD9 and CD81 but not with other tetraspanins or with integrins. Immunodepletion experiments indicated that EWI-2-CD9/CD81 interactions are highly stoichiometric, ... More
The CD9 molecule on stromal cells.
AuthorsHayashi S, Miyake K, Kincade PW
JournalLeuk Lymphoma
PubMed ID10830733
Numerous functions have been attributed to CD9 and other members of the transmembrane 4 (TM4) superfamily. CD9 is thought to be involved in cell proliferation, differentiation, motility and survival. It may also function as part of toxin and virus receptor complexes. Although much remains to be learned about molecular mechanisms, ... More
Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion.
AuthorsHigginbottom A, Takahashi Y, Bolling L, Coonrod SA, White JM, Partridge LJ, Monk PN
JournalBiochem Biophys Res Commun
PubMed ID14575715
CD9 has been shown to be essential for sperm/oocyte fusion in mice, the only non-redundant role found for a member of the tetraspanin family. CD9 can act in cis, reconstituting sperm/oocyte fusion when ectopically expressed in oocytes from CD9 null mice, or in trans, inhibiting sperm fusion when the large ... More
Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking.
AuthorsKovalenko OV, Yang X, Kolesnikova TV, Hemler ME
JournalBiochem J
PubMed ID14556650
It is a well-established fact that tetraspanin proteins, a large family of integral membrane proteins involved in cell motility, fusion and signalling, associate extensively with one another and with other transmembrane and membrane-proximal proteins. In this study, we present results strongly suggesting that tetraspanin homodimers are fundamental units within larger ... More