DMEM/F-12, HEPES, no phenol red
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DMEM/F-12, HEPES, no phenol red
Citations & References (3)
Gibco™

DMEM/F-12, HEPES, no phenol red

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many differentRead more
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Catalog NumberQuantity
11039021500 mL
1103904710 x 500 mL
Catalog number 11039021
Price (CNY)
653.20
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Ends: 31-Dec-2025
833.00
Save 179.80 (22%)
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Quantity:
500 mL
Customize this product
Price (CNY)
653.20
Online Exclusive
Ends: 31-Dec-2025
833.00
Save 179.80 (22%)
Each
Add to cart

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With: L-glutamine, HEPES

Without: Phenol Red

The complete formulation is available.

Using DMEM/F-12

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineMDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Concentration1X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeDMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Glutamine, HEPES
Without AdditivesNo Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM/F-12, HEPES, no phenol red, 500 mL FAQs

Citations & References (3)

Citations & References
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Identification of a contractile-responsive element in the cardiac alpha-myosin heavy chain gene.
Authors: Ojamaa K; Samarel A M; Klein I;
Journal:J Biol Chem
PubMed ID:8537395
The mechanisms by which the cardiac-specific alpha-myosin heavy chain (alpha-MHC) gene responds to contractile activity was studied in cultured cardiomyocytes and in vivo. Deletion analysis of the alpha-MHC promoter transiently transfected into neonatal rat cardiomyocytes localized the contractile-responsive element within -80 to -40 base pairs of the transcriptional start site. ... More
Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair.
Authors:Sountoulidis A, Stavropoulos A, Giaglis S, Apostolou E, Monteiro R, Chuva de Sousa Lopes SM, Chen H, Stripp BR, Mummery C, Andreakos E, Sideras P
Journal:PLoS One
PubMed ID:22916109
Signaling by Bone Morphogenetic Proteins (BMP) has been implicated in early lung development, adult lung homeostasis and tissue-injury repair. However, the precise mechanism of action and the spatio-temporal pattern of BMP-signaling during these processes remains inadequately described. To address this, we have utilized a transgenic line harboring a BMP-responsive eGFP-reporter ... More