Dynabeads™ Human T-Activator CD3/CD28/CD137

Benefits of Dynabeads Human T-Activator CD3/CD28/CD137 beads include:

• Easy—no need to prepare antigen-presenting cells or antigen
• Efficient—improved expansion and increased survival of Ag-specific T-cells
• Functional—expanded T cells maintain Ag-specific responses

Simple and fast procedure

Dynabeads Human T-Activator CD3/CD28/CD137 beads offer a simple method for expansion of CD3+, CD4+, or CD8+ antigen-specific T cells from T cell clones, cell lines, or freshly isolated Ag-specific T cells from PBMC or whole blood. Ag-specific T cells are activated by adding the beads and human recombinant IL-2 (rIL-2) and rIL7 (for CD8+ Ag-specific T cells only) to the cell culture. Optimal expansion is performed when appropriate T-cell medium is used, such as CTS OpTmizer T Cell Expansion SFM or Advanced RPMI 1640 Medium with added supplements according to the manual.

When experiencing reduced expansion or cell shrinking the T cells can be restimulated with the Dynabeads Human T-Activator CD3/CD28/CD137 beads. The beads can be removed with a DynaMag magnet and used in applications like cell:cell interaction studies, flow cytometry (after 3–4 days of culture to obtain bead release), proteomics, or nucleic acid-based assays. The magnetic beads in this product may not appear as a typical brown suspension and may not be easily visible. This is normal and does not affect performance. Be sure to thoroughly resuspend before use.

Dynabeads Human T-Activator CD3/CD28/CD137 applications

The use of Dynabeads Human T-Activator CD3/CD28/CD137 beads have also been used to expand chronic lymphocytic leukemia in vitro by activating T cells, which in turn promote the expansion of leukemic B cells (Baptista et al, 2021). In addition, the Dynabeads Human T-Activator CD3/CD28/CD137 beads have been used to understand T cell suppression in blood neoplasms (Deng, et al, 2018).

Pre-isolate human antigen-specific T cells

To obtain the most optimal Ag-specific expansion from a heterogenous T-cell population, the Ag-specific T cells can be sorted or isolated and released directly from human whole blood or from peripheral blood mononuclear cells (PBMCs) with the Dynabeads FlowComp Flexi Kit in combination with an agonistic anti-CD137 antibody.

Alternative activation products

• For activation of naïve polyclonal human T cells, use Dynabeads Human T Activator CD3/CD28 (Cat. No. 11131D)
• For activation of naïve polyclonal mouse T cells, use Dynabeads Mouse T Activator CD3/CD28 (Cat. No. 11456D)
• For activation of human regulatory T cells (Tregs), use Dynabeads CD3/CD28 Treg Expander (Cat. No. 11129D)
• For ex vivo activation and expansion of human T cells for cell-based therapy, use the aseptically manufactured CTS Dynabeads CD3/CD28 (Cat. No. 40203D)

Commercial supply

Our manufacturing sites are ISO 13485–certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility, making them an ideal choice for commercial supply. If you are utilizing Dynabeads Human T-Activator CD3/CD28/CD137 beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com.

References

Deng M, et al., LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018 Oct;562(7728):605-609.

Baptista MJ, Baskar S, Gaglione EM, Keyvanfar K, Ahn IE, Wiestner A, Sun C. Select Antitumor Cytotoxic CD8+ T Clonotypes Expand in Patients with Chronic Lymphocytic Leukemia Treated with Ibrutinib. Clin Cancer Res. 2021 Aug 15;27(16):4624-4633.