View additional product information for Dynabeads™ Streptavidin Magnetic Beads - Citations (65604D, 65801D, 65605D, 65002, 65001, 65602, 65305, 65601, 11205D, 11206D, 65306, 65606D, 65607D, 60101, 60210)
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Citations & References
Abstract
Detection of tyrosinase mRNA in melanoma by reverse transcription-PCR and electrochemiluminescence.
AuthorsO'Connell CD, Juhasz A, Kuo C, Reeder DJ, Hoon DS,
JournalClin Chem
PubMed ID9625038
Increased sensitivity and improved quantitation of analytical tests used in biotechnology and clinical chemistry are goals of many laboratories. We have used tyrosinase primers to specifically amplify by RT-PCR the tyrosinase mRNA expressed by the M12 melanoma cell line in a background of mRNA from breast cancer cells. An electrochemiluminescence ... More
AID-induced decrease in topoisomerase 1 induces DNA structural alteration and DNA cleavage for class switch recombination.
AuthorsKobayashi M, Aida M, Nagaoka H, Begum NA, Kitawaki Y, Nakata M, Stanlie A, Doi T, Kato L, Okazaki IM, Shinkura R, Muramatsu M, Kinoshita K, Honjo T,
JournalProc Natl Acad Sci U S A
PubMed ID20018730
'To initiate class switch recombination (CSR) activation-induced cytidine deaminase (AID) induces staggered nick cleavage in the S region, which lies 5'' to each Ig constant region gene and is rich in palindromic sequences. Topoisomerase 1 (Top1) controls the supercoiling of DNA by nicking, rotating, and religating one strand of DNA. ... More
Recommended mass spectrometry-based strategies to identify botulinum neurotoxin-containing samples.
AuthorsKalb SR, Baudys J, Wang D, Barr JR
Journal
PubMed ID25996606
'Botulinum neurotoxins (BoNTs) cause the disease called botulism, which can be lethal. BoNTs are proteins secreted by some species of clostridia and are known to cause paralysis by interfering with nerve impulse transmission. Although the human lethal dose of BoNT is not accurately known, it is estimated to be between ... More
Using phage and yeast display to select hundreds of monoclonal antibodies: application to antigen 85, a tuberculosis biomarker.
'Current diagnostic methods for tuberculosis (TB), a major global health challenge that kills nearly two million people annually, are time-consuming and inadequate. During infection a number of bacterial molecules that play a role in the infective process are released and have been proposed as biomarkers for early TB diagnosis. Antigen ... More
PCR-free detection of genetically modified organisms using magnetic capture technology and fluorescence cross-correlation spectroscopy.
AuthorsZhou X, Xing D, Tang Y, Chen WR,
JournalPLoS One
PubMed ID19956680
'The safety of genetically modified organisms (GMOs) has attracted much attention recently. Polymerase chain reaction (PCR) amplification is a common method used in the identification of GMOs. However, a major disadvantage of PCR is the potential amplification of non-target DNA, causing false-positive identification. Thus, there remains a need for a ... More
PK assays for antibody-drug conjugates: case study with ado-trastuzumab emtansine.
AuthorsDere R, Yi JH, Lei C, Saad OM, Huang C, Li Y, Baudys J, Kaur S
JournalBioanalysis
PubMed ID23641694
Antibody-drug conjugates (ADCs) combine the characteristics of large-molecule biologics and small-molecule drugs and are heterogeneous mixtures that can biotransform in vivo, resulting in additional complexity. ADC bioanalytical strategies require novel analytical methods, as well as existing large- and small-molecule methods. Because ADCs in late-stage clinical development are relatively new, regulatory ... More
Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing.
AuthorsGnirke A, Melnikov A, Maguire J, Rogov P, LeProust EM, Brockman W, Fennell T, Giannoukos G, Fisher S, Russ C, Gabriel S, Jaffe DB, Lander ES, Nusbaum C,
JournalNat Biotechnol
PubMed ID19182786
Targeting genomic loci by massively parallel sequencing requires new methods to enrich templates to be sequenced. We developed a capture method that uses biotinylated RNA 'baits' to fish targets out of a 'pond' of DNA fragments. The RNA is transcribed from PCR-amplified oligodeoxynucleotides originally synthesized on a microarray, generating sufficient ... More
Nanowire-transistor based ultra-sensitive DNA methylation detection.
Accurate detection of DNA methylation at specific gene transcription sites is important to identify potential tumor formation since this epigenetic alteration may result in silencing of tumor suppressor genes that protect against tumor formation or that repair damaged DNA. Current technologies used in DNA methylation detection are complicated and time ... More
Regulation of Op18 during spindle assembly in Xenopus egg extracts.
AuthorsBudde PP, Kumagai A, Dunphy WG, Heald R,
JournalJ Cell Biol
PubMed ID11285281
Oncoprotein 18 (Op18) is a microtubule-destabilizing protein that is negatively regulated by phosphorylation. To evaluate the role of the three Op18 phosphorylation sites in Xenopus (Ser 16, 25, and 39), we added wild-type Op18, a nonphosphorylatable triple Ser to Ala mutant (Op18-AAA), and to mimic phosphorylation, a triple Ser to ... More
Non-SELEX: selection of aptamers without intermediate amplification of candidate oligonucleotides.
AuthorsBerezovski MV, Musheev MU, Drabovich AP, Jitkova JV, Krylov SN
JournalNat Protoc
PubMed ID17406423
Aptamers are typically selected from libraries of random DNA (or RNA) sequences through systematic evolution of ligands by exponential enrichment (SELEX), which involves several rounds of alternating steps of partitioning of candidate oligonucleotides and their PCR amplification. Here we describe a protocol for non-SELEX selection of aptamers--a process that involves ... More
Validation of the Endopep-MS method for qualitative detection of active botulinum neurotoxins in human and chicken serum.
AuthorsBjörnstad K, Tevell Åberg A, Kalb SR, Wang D, Barr JR, Bondesson U, Hedeland M
Journal
PubMed ID25228079
Botulinum neurotoxins (BoNTs) are highly toxic proteases produced by anaerobic bacteria. Traditionally, a mouse bioassay (MBA) has been used for detection of BoNTs, but for a long time, laboratories have worked with alternative methods for their detection. One of the most promising in vitro methods is a combination of an ... More
Detection of Botulinum Toxins A, B, E, and F in Foods by Endopep-MS.
AuthorsKalb SR, Krilich JC, Dykes JK, Lúquez C, Maslanka SE, Barr JR
Journal
PubMed ID25578960
Botulism is caused by exposure to botulinum neurotoxins (BoNTs). BoNTs are proteins secreted by some species of clostridia; these neurotoxins are known to interfere with nerve impulse transmission, thus causing paralysis. Botulism may be contracted through consumption of food either naturally or intentionally contaminated with BoNT. The human lethal dose ... More
Functional characterization of botulinum neurotoxin serotype H as a hybrid of known serotypes F and A (BoNT F/A).
AuthorsKalb SR, Baudys J, Raphael BH, Dykes JK, Lúquez C, Maslanka SE, Barr JR
Journal
PubMed ID25731972
A unique strain of Clostridium botulinum (IBCA10-7060) was recently discovered which produces two toxins: botulinum neurotoxin (BoNT) serotype B and a novel BoNT reported as serotype H. Previous molecular assessment showed that the light chain (LC) of the novel BoNT most resembled the bont of the light chain of known ... More
Generation and Characterization of an IgG4 Monomeric Fc Platform.
AuthorsShan L, Colazet M, Rosenthal KL, Yu XQ, Bee JS, Ferguson A, Damschroder MM, Wu H, Dall'Acqua WF, Tsui P, Oganesyan V
JournalPLoS One
PubMed ID27479095
The immunoglobulin Fc region is a homodimer consisted of two sets of CH2 and CH3 domains and has been exploited to generate two-arm protein fusions with high expression yields, simplified purification processes and extended serum half-life. However, attempts to generate one-arm fusion proteins with monomeric Fc, with one set of ... More
A U1 snRNP-specific assembly pathway reveals the SMN complex as a versatile hub for RNP exchange.
AuthorsSo BR, Wan L, Zhang Z, Li P, Babiash E, Duan J, Younis I, Dreyfuss G
JournalNat Struct Mol Biol
PubMed ID26828962
Despite equal snRNP stoichiometry in spliceosomes, U1 snRNP (U1) is typically the most abundant vertebrate snRNP. Mechanisms regulating U1 overabundance and snRNP repertoire are unknown. In Sm-core assembly, a key snRNP-biogenesis step mediated by the SMN complex, the snRNA-specific RNA-binding protein (RBP) Gemin5 delivers pre-snRNAs, which join SMN-Gemin2-recruited Sm proteins. ... More
Rapid quantification of a cleavable antibody-conjugated drug by liquid chromatography/tandem mass spectrometry with microwave-assisted enzymatic cleavage.
AuthorsXu L, Packer LE, Qian MG, Wu JT
JournalJ Pharm Biomed Anal
PubMed ID27281578
Antibody-drug conjugates (ADCs) play an increasingly important role for targeted cancer treatment. One class of ADCs has attracted particular interest in drug development. These ADCs employ a cleavable chemistry linkage for drugs and utilize the reduced interchain disulfide cysteine residues for conjugation. In this work, a novel bioanalytical method for ... More