CD 杂交瘤培养基

CD 杂交瘤培养基是一种非动物源性、化学成分明确的无蛋白培养基，专门开发用于支持杂交瘤的生长以生产抗体。–Gibco™ CD 杂交瘤培养基特点：•可支持悬浮和固定杂交瘤和骨髓瘤•为获得稳定性，未使用 L-谷氨酰胺配制•非动物源性、无蛋白、化学成分明确的配方–可支持悬浮和固定杂交瘤和骨髓瘤适于培养重组骨髓瘤细胞系以及传统杂交瘤，Gibco™ CD了解更多信息

货号	数量
11279023	1000 mL

货号 11279023

价格（CNY)

1,937.00

添加至购物车

1000 mL

Customize this product

价格（CNY)

1,937.00

添加至购物车

CD 杂交瘤培养基是一种非动物源性、化学成分明确的无蛋白培养基，专门开发用于支持杂交瘤的生长以生产抗体。–Gibco™ CD 杂交瘤培养基特点：

•可支持悬浮和固定杂交瘤和骨髓瘤
•为获得稳定性，未使用 L-谷氨酰胺配制
•非动物源性、无蛋白、化学成分明确的配方–

可支持悬浮和固定杂交瘤和骨髓瘤
适于培养重组骨髓瘤细胞系以及传统杂交瘤，Gibco™ CD 杂交瘤培养基可提供高细胞密度和 IgG 生产。胆固醇依赖性培养物（如 NS0）需要添加 250X 胆固醇脂质浓缩液。

为获得稳定性，未使用 L-谷氨酰胺配制
Gibco™ CD 杂交瘤培养基在使用前需要添加 8 mM L-谷氨酰胺或 GlutaMAX™ 补充剂。GlutaMAX™ 补充剂可尽可能减少有毒氨的积聚，并可改善细胞活力和生长（易于使用的规格）。

非动物源性–、无蛋白且化学成分明确的配方
Gibco™ CD 杂交瘤培养基为非动物源性–、无蛋白且化学成分明确，可以更轻松地纯化您的目标蛋白。Gibco™ 化学成分明确的培养基不含蛋白、水解物或未知成分的组分。

产品使用
在生产过程中使用 Gibco™ CD 杂交瘤培养基并已向 FDA 提交申请的客户可以向我们索取授权函以便引用我们的 II 型药物主文件 (DMF)。

cGMP 生产和质量系统
Gibco™ CD 杂交瘤培养基在位于 Grand Island（纽约）的符合 cGMP 要求的工厂内生产。该工厂是在FDA登记的医疗器械生产商，且通过ISO 13485标准认证。

仅用于研究和生产用途。不可用于临床诊断或直接用于人类或动物。

细胞类型骨髓瘤细胞、杂交瘤

产品线Gibco™

产品类型杂交瘤培养基

数量1000 mL

运输条件室温

分类非动物源性, 化学成分明确, 无蛋白

不加添加剂无谷氨酰胺, 无酚红

Unit SizeEach

内容与储存

储存条件：2-8°C。避光储存
运输条件：环境条件
有效期：自生产之日起 18 个月

常见问题解答 (FAQ)

How do I adapt my cells to serum-free medium?

Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.

SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

Why is it necessary to gradually adapt the cells to serum-free medium?

Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I filter sterilize CD Hybridoma Medium?

CD Hybridoma Medium can be filtered by 0.2 µm filtration.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.