TC酵母粉:1.66 g,乳清蛋白水解物1.66 g,L-谷氨酰胺:0.3 g
这些添加剂能够帮助增加培养基的稳定性和延长保质期。请注意这些添加剂未经消毒,须经过滤灭菌(通过0.2 µm滤器)后方可加入。
TNM-FH未加入热灭活的10% FBS之前,不视为完全培养基。血清会提供额外的养分,同时也能够保护悬浮培养条件(即旋转器和振荡培养瓶)下的细胞免受流体压力的影响。
What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?
Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the base growth medium. For the complete growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This complete growth medium is also referred to as TNM-FH.