Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples.
AuthorsSmith AM, Heisler LE, St Onge RP, Farias-Hesson E, Wallace IM, Bodeau J, Harris AN, Perry KM, Giaever G, Pourmand N, Nislow C,
JournalNucleic Acids Res
PubMed ID20460461
'Next-generation sequencing has proven an extremely effective technology for molecular counting applications where the number of sequence reads provides a digital readout for RNA-seq, ChIP-seq, Tn-seq and other applications. The extremely large number of sequence reads that can be obtained per run permits the analysis of increasingly complex samples. For ... More
The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma.
AuthorsDesRochers TM, Shamis Y, Alt-Holland A, Kudo Y, Takata T, Wang G, Jackson-Grusby L, Garlick JA,
JournalEpigenetics
PubMed ID22207358
The microenvironment plays a significant role in human cancer progression. However, the role of the tumor microenvironment in the epigenetic control of genes critical to cancer progression remains unclear. As transient E-cadherin expression is central to many stages of neoplasia and is sensitive to regulation by the microenvironment, we have ... More
Mutations in the RNase H domain of HIV-1 reverse transcriptase affect the initiation of DNA synthesis and the specificity of RNase H cleavage in vivo.
Authors Julias John G; McWilliams Mary Jane; Sarafianos Stefan G; Arnold Edward; Hughes Stephen H;
JournalProc Natl Acad Sci U S A
PubMed ID12093908
Retroviral reverse transcriptases contain a DNA polymerase activity that can copy an RNA or DNA template and an RNase H activity that degrades the viral RNA genome during reverse transcription. RNase H makes both specific and nonspecific cleavages; specific cleavages are used to generate and remove the polypurine tract primer ... More
Integration of the Non-genomic and Genomic Actions of Estrogen. MEMBRANE-INITIATED SIGNALING BY STEROID TO TRANSCRIPTION AND CELL BIOLOGY.
Authors Pedram Ali; Razandi Mahnaz; Aitkenhead Mark; Hughes Christopher C W; Levin Ellis R;
JournalJ Biol Chem
PubMed ID12372818
Estrogen binds to receptors that translocate to the plasma membrane and to the nucleus. The rapid, non-genomic actions of this sex steroid are attributed to membrane action, while gene transcription occurs through nuclear receptor function. However, gene transcription can also result from estrogen signaling initiated at the membrane, but the ... More