Does the ProQuest Two-Hybrid System yeast strain, MaV203 have slow growth compared to other commonly used laboratory Saccharomyces cerevisiae strains?
To avoid interference of endogenous GAL4 and GAL80 proteins, MaV203 must carry deletions of the GAL4 and GAL80 genes. As a result of deletion of these two genes, MaV203 cells grow more slowly compared to yeast strains containing the wild-type version of these genes. Please note that the growth rate will depend on the type of growth medium used (e.g., YPD versus SC drop-out media).
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Are the MaV203 yeast cells that come with the ProQuest Two-Hybrid System ready for transformation?
No, the MaV203 cells are provided as a glycerol stock. Cells from this stock need to be streaked on a YPD plate for growth at 30 degrees C. Resulting colonies can be used to prepare competent cells for transformation as per the ProQuest Two-Hybrid System manual. Alternatively, ready-to-use MaV203 Competent Yeast Cells, Subcloning Scale (Cat. No. 11445-012) or Library Scale (Cat. No. 11281-011) are available for purchase from Thermo Fisher Scientific.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?
Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.
Are your vectors routinely sequenced?
No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.
How was the reference sequence for your vectors created?
Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.
