Platinum™ GenoType Tsp DNA 聚合酶
Platinum&trade; GenoType <i>Tsp</i> DNA 聚合酶
Invitrogen™

Platinum™ GenoType Tsp DNA 聚合酶

Platinum™ GenoType Tsp DNA 聚合酶专门为基于 PCR 的基因分型应用中二核苷酸重复序列位点的扩增而设计和进行质量认定。该酶与包含抗 Tsp DNA了解更多信息
Have Questions?
更改视图buttonViewtableView
货号反应次数
11448032
又称 11448-032
2,000 次反应
11448024400 次反应
货号 11448032
又称 11448-032
价格(CNY)
32,095.00
Each
添加至购物车
反应次数:
2,000 次反应
价格(CNY)
32,095.00
Each
添加至购物车
Platinum™ GenoType Tsp DNA 聚合酶专门为基于 PCR 的基因分型应用中二核苷酸重复序列位点的扩增而设计和进行质量认定。该酶与包含抗 Tsp DNA 聚合酶单克隆抗体的耐热抑制剂预先形成复合物,从而提供一种可提高 PCR 特异性、允许在室温下准备 PCR 反应体系的自动化“热启动”方法。Platinum™ GenoType Tsp DNA 聚合酶:

•表现出向 PCR 产物添加极少的非模板化核苷酸
• 缺少 5´ 和 3´ 核酸外切酶活性
• 将片段扩增至 500 bp

应用
在消除非模板化核苷酸添加的应用中,二核苷酸重复位点扩增是可取的。

单位定义
在标准的 Taq DNA 聚合酶反应条件下的二核苷酸重复位点扩增中,一个单位的 Platinum™ GenoType Tsp DNA 聚合酶被确定为在功能上与一个单位的 Taq DNA 聚合酶等效。一单位 Tsp DNA 聚合酶近似2.5活性单位。在优化的反应条件下(74°C 时),一个活性单位会在30分钟内将 10 nmol 脱氧核苷酸掺入酸沉淀材料中。
仅供科研使用。不可用于诊断程序。
规格
保真度(相对于 Taq)1 X
热启动内置热启动
反应次数2,000 次反应
突出端平末端
聚合酶Tsp 基因分型聚合酶
数量2500 单位
反应形式分离组分
运输条件湿冰或干冰
尺寸(最终产品)500 bp 或更小
容量500 μL
适用于(应用)Hot-start PCR
高 GC PCR 扩增效果
反应速度标准
Unit SizeEach
内容与储存
目录:
• 1管 500 μL (5 Tsp U/μL)
• 10X PCR 缓冲液 (200mM Tris-HCl, pH 8.4, 500 mM KCl))
• 1瓶 50 mM MgCl2

-20°C 储存。妥善储存时,稳定性至少可保持6个月。

常见问题解答 (FAQ)

Platinum 技术 和 AccuPrime 技术有何差异?

使用Platinum 技术时,在94°C的变性步骤之前,抗DNA聚合酶的抗体结合在酶上。在变性步骤中,抗体将随之降解,聚合酶活化,引物开始介导延伸。AccuPrime Taq将PlatinumTaq (Taq + Platinum抗体) 与我们专利的热稳定 AccuPrime 辅助蛋白相结合。10x反应缓冲液中包含了该辅助蛋白,以提高每轮PCR反应中引物和模板结合的特异性。

What is the difference between Platinum technology and AccuPrime technology?

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Is there anything to prevent AmpliTaq Gold DNA polymerase from extending from the 3’ end of a TaqMan probe in a 5’ nuclease assay?

Yes. There is a phosphate group on the 3' end of all TaqMan probes that prevents such extension.

How does AmpliTaq Gold DNA Polymerase differ from AmpliTaq DNA Polymerase?

AmpliTaq Gold DNA Polymerase is a modified form of AmpliTaq DNA Polymerase that contains a proprietary chemical (or so-called hot start molecule) bound to the enzyme's active site. In order to activate the AmpliTaq Gold DNA Polymerase fully, we recommend an initial activation step of 95 degrees C for 10 min when using GeneAmp 10X PCR Buffer I and/or GeneAmp 10X PCR Buffer II and Mg in one of our thermal cyclers. When using GeneAmp 10X PCR Gold Buffer, activation time can be reduced to 5 minutes. Once activation is complete, you can proceed with your standard PCR cycling program (denaturing, annealing, extension, etc).

Does AmpliTaq Gold DNA Polymerase contain exonuclease (proofreading) activity?

No, AmpliTaq Gold DNA polymerase does not contain proofreading activity, however fidelity in PCR amplifications utilizing this enzyme may be improved. High fidelity can be achieved by: 1. Decreasing the final concentration of each nucleotide to 40-50 uM. 2. Using the lowest MgCl2 concentration possible. 3. Using less enzyme. 4. Decreasing extension times. 5. Using the highest annealing temperature possible. 6. Using as few cycles as possible.