Newly identified stress-responsive protein kinases, Krs-1 and Krs-2.
AuthorsTaylor LK, Wang HC, Erikson RL
JournalProc Natl Acad Sci U S A
PubMed ID8816758
'The activation of protein kinases is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. However, when several agents result in the activation of the same enzymes, it is unclear how specific biological responses are generated. We describe here two protein kinases that ... More
A conserved MST-FOXO signaling pathway mediates oxidative-stress responses and extends life span.
AuthorsLehtinen MK, Yuan Z, Boag PR, Yang Y, Villén J, Becker EB, DiBacco S, de la Iglesia N, Gygi S, Blackwell TK, Bonni A,
JournalCell
PubMed ID16751106
Oxidative stress influences cell survival and homeostasis, but the mechanisms underlying the biological effects of oxidative stress remain to be elucidated. Here, we demonstrate that the protein kinase MST1 mediates oxidative-stress-induced cell death in primary mammalian neurons by directly activating the FOXO transcription factors. MST1 phosphorylates FOXO proteins at a ... More
Cloning and characterization of a human protein kinase with homology to Ste20.
AuthorsCreasy CL, Chernoff J
JournalJ Biol Chem
PubMed ID7665586
A human protein kinase (termed MST1) has been cloned and characterized. The MST1 catalytic domain is most homologous to Ste20 and other Ste20-like kinases (62-65% similar). MST1 is expressed ubiquitously, and the MST1 protein is present in all human cell lines examined. Biochemical characterization of MST1 catalytic activity demonstrates that ... More
Role of the tumor suppressor RASSF1A in Mst1-mediated apoptosis.
AuthorsOh HJ, Lee KK, Song SJ, Jin MS, Song MS, Lee JH, Im CR, Lee JO, Yonehara S, Lim DS
JournalCancer Res
PubMed ID16510573
Mammalian sterile 20-like kinase 1 (Mst1) is activated by both caspase-mediated cleavage and phosphorylation in response to apoptotic stimuli, including Fas ligation. Here, we examined the possible role of the tumor suppressor RASSF1A in Mst1 activation and Mst1-mediated apoptosis induced by death receptor signaling. Immunoprecipitation and immunofluorescence analyses revealed that ... More
The Ste20-like protein kinase, Mst1, dimerizes and contains an inhibitory domain.
AuthorsCreasy CL, Ambrose DM, Chernoff J
JournalJ Biol Chem
PubMed ID8702870
The human serine/threonine protein kinases, Mst1 and Mst2, share considerable homology to Ste20 and p21-activated kinase (Pak) throughout their catalytic domains. However, outside the catalytic domains there are no significant homologies to previously described Ste20-like kinases or other proteins. To understand the role of the nonhomologous regions, we performed a ... More