Grace 昆虫培养基，未补充
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Gibco™

Grace 昆虫培养基，未补充

Grace 昆虫培养基最初设计用于澳大利亚白星橙天蚕蛾（桉树柞蚕）的细胞生长。补充的 Grace 未补充昆虫培养基目前广泛用于草地贪夜蛾细胞 Sf9 和 Sf21了解更多信息
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货号数量
11595030500 mL
货号 11595030
价格（CNY)
1,789.00
Each
添加至购物车
数量:
500 mL
Customize this product
价格（CNY)
1,789.00
Each
添加至购物车
Grace 昆虫培养基最初设计用于澳大利亚白星橙天蚕蛾（桉树柞蚕）的细胞生长。补充的 Grace 未补充昆虫培养基目前广泛用于草地贪夜蛾细胞 Sf9 和 Sf21 的生长。Sf9 和 Sf21 细胞通常用于借助杆状病毒表达载体系统 (BEVS) 来表达重组蛋白。Gibco™ Grace 未添加昆虫培养基可用于昆虫细胞转染或蛋白标记研究（含蛋氨酸）。针对广泛的昆虫细胞培养应用，我们提供了多种 Gibco™基质选项。

这种 Grace 昆虫培养基经过如下改良：

包含不含
•L-谷氨酰胺 • 乳清蛋白水解物
•碳酸氢钠 • 酵母粉
• 蛋氨酸

完整的配方请见此处。Grace 未补充昆虫培养基不含补充培养基中常见的乳清蛋白水解物和酵母粉。Gibco™ Grace 未补充昆虫培养基也不含蛋氨酸，用于蛋白标记研究。

产品用途
仅供研究使用：不适用于动物或人诊断或治疗用途。

cGMP 生产和质量体系
Gibco™ Grace 未补充昆虫培养基在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在 FDA 注册的医疗器械生产商，且通过 ISO 13485 标准认证。

在用作生长培养基之前，Grace 未补充昆虫培养基需要补充 10% 胎牛血清 (FBS)、50 mg/L 蛋氨酸、3.33 g/L 乳清蛋白水解物和 3.33 g/L 酵母粉。Grace 非补充昆虫培养基需要环境 CO2 来维持生理 pH 值。
仅供科研使用。不可用于诊断程序。
规格
细胞系Sf21, Sf9
细胞类型昆虫细胞
无机盐钙、镁
产品线Gibco
产品类型Grace 昆虫培养基
数量500 mL
运输条件室温
形式液体
加有添加剂谷氨酰胺, 碳酸氢钠
不加添加剂无乳清蛋白水解物, 无酵母, 不含蛋氨酸
Unit SizeEach
内容与储存
储存条件：2-8°C。避光储存
运输条件：环境条件
有效期：自生产之日起 12 个月

常见问题解答 (FAQ)

Grace’s昆虫培养基中包含了哪些辅剂？为什么它们以单管形式提供？

补充培养基简称为TNM-FH。它包含以下成份（每500 mL）：

TC酵母粉：1.66 g，乳清蛋白水解物1.66 g，L-谷氨酰胺：0.3 g
这些添加剂能够帮助增加培养基的稳定性和延长保质期。请注意这些添加剂未经消毒，须经过滤灭菌（通过0.2 µm滤器）后方可加入。
TNM-FH未加入热灭活的10% FBS之前，不视为完全培养基。血清会提供额外的养分，同时也能够保护悬浮培养条件（即旋转器和振荡培养瓶）下的细胞免受流体压力的影响。

你们是否为杆状病毒表达应用提供不含甲硫氨酸的培养基？

是的，我们提供不含甲硫氨酸的Grace’s昆虫培养基（货号11595030）。

 

For how long can I store Grace's Insect Medium, unsupplemented at room temperature?

We ship this product under “ambient” conditions throughout the year. We have performed shipping studies that shown components to be stable for at least 7 days at ambient temperatures. The important factor is to ensure that the media is not exposed to light at the warmer temperatures as there are vitamins and amino acids that will breakdown with light exposure.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer a methionine-free media for baculovirus expression?

Yes, we offer our methionine-free Grace's Insect Medium (Cat. No. 11595030).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (2)

引用和文献
Abstract
Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization.
Authors:Xu L, Harada H, Yokohama-Tamaki T, Matsumoto S, Tanaka J, Taniguchi A,
Journal:J Biol Chem
PubMed ID:16293627
'Amelogenin is an extracellular matrix protein secreted by ameloblasts and is a major component of enamel matrix. Recently, in addition to their role in enamel formation, the biological activity of enamel proteins in the process of cell differentiation has recently become widely appreciated. In this study, we examined the biological ... More
A serine/threonine-rich motif is one of three nuclear localization signals that determine unidirectional transport of the mineralocorticoid receptor to the nucleus.
Authors:Walther RF, Atlas E, Carrigan A, Rouleau Y, Edgecombe A, Visentin L, Lamprecht C, Addicks GC, Haché RJ, Lefebvre YA,
Journal:J Biol Chem
PubMed ID:15737989
The mineralocorticoid receptor (MR) is a tightly regulated nuclear hormone receptor that selectively transmits corticosteroid signals. Steroid treatment transforms MR from a transcriptionally inert state, in which it is distributed equally between the nucleus and cytoplasm, to an active completely nuclear transcription factor. We report here that MR is an ... More