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Invitrogen™
Taq DNA 聚合酶（巴西）
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa.
| 货号 | 反应次数 |
|---|---|
| 11615010 | 500 次反应 |
货号 11615010
价格（CNY)
954.00
Each
反应次数:
500 次反应
价格（CNY)
954.00
Each
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity (see figure).
Taq DNA Polymerase native enzyme is purified from Thermus aquaticus YT1. Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
Features
- Choice of recombinant or native enzyme
- Amplification of PCR products up to 5 kb in size
- Enzyme is licensed and qualified for PCR
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- Sequencing ssDNA
- Cycle sequencing
Notes
- One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.
仅供科研使用。不可用于诊断程序。
规格
保真度（相对于 Taq）1X
热启动否
反应次数500 次反应
突出端3'-A
聚合酶Taq DNA Polymerase
数量500 单位
运输条件干冰
尺寸（最终产品）5 kb或更小
原始材料DNA
最大浓度5 U/μL
适用于（应用）RT-PCR
高 GC PCR 扩增效果低
反应速度标准
Unit SizeEach
内容与储存
3 管，储存在 -20°C 下