Search

  • 联系我们
  • 货号直购

Grace's Insect Medium (2X), supplemented - FAQs

查看更多产品信息 Grace's Insect Medium (2X), supplemented - FAQs (11667037)

4 个常见问题解答

Grace’s昆虫培养基中包含了哪些辅剂？为什么它们以单管形式提供？

补充培养基简称为TNM-FH。它包含以下成份（每500 mL）：

TC酵母粉：1.66 g，乳清蛋白水解物1.66 g，L-谷氨酰胺：0.3 g
这些添加剂能够帮助增加培养基的稳定性和延长保质期。请注意这些添加剂未经消毒，须经过滤灭菌（通过0.2 µm滤器）后方可加入。
TNM-FH未加入热灭活的10% FBS之前，不视为完全培养基。血清会提供额外的养分，同时也能够保护悬浮培养条件（即旋转器和振荡培养瓶）下的细胞免受流体压力的影响。

为了开展噬斑测定实验，你们推荐使用何种培养基来培育Sf9或Sf21细胞？

我们推荐您使用我们的Grace’s supplemented昆虫培养基（货号11667037）或SF-900培养基（1.3X）（货号10967032）。
Grace’s Supplemented昆虫培养基是2X的配方培养基，专为在噬斑测定实验琼脂糖覆盖层中的应用而配制。本培养基经以下成份的微调：
乳清蛋白水解物
酵母粉
L-谷氨酰胺
碳酸氢钠
点击此处(https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html)获取完整配方。

SF-900培养基（1.3X）是为Sf9和Sf21细胞准备的不含血清的低蛋白昆虫细胞培养基，适用于在噬斑测定实验1%的琼脂糖覆盖层中使用。

What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What medium do you recommend to grow Sf9 or Sf21 cells for performing plaque assays?

We would recommend using our Grace's Supplemented Insect Medium (Cat. No. 11667037) or SF-900 Medium (1.3X) (Cat. No. 10967032). Grace's Supplmented Insect Media is a 2X formulation intended for use in the agarose overlay when performing the plaque assay. The media has been modified with:
- Lactalbumin hydrolysate
- Yeastolate
- L-glutamine
- Sodium bicarbonate

Please go here for the complete formulation (http://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html). SF-900 Medium (1.3X) is a complete serum-free low protein insect cell culture medium for Sf9 and Sf21 cells, which can be used for 1% agarose overlays used for plaque assays.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.