Grace 补充昆虫培养基 (2X)

补充培养基简称为TNM-FH。它包含以下成份（每500 mL）：

TC酵母粉：1.66 g，乳清蛋白水解物1.66 g，L-谷氨酰胺：0.3 g
这些添加剂能够帮助增加培养基的稳定性和延长保质期。请注意这些添加剂未经消毒，须经过滤灭菌（通过0.2 µm滤器）后方可加入。
TNM-FH未加入热灭活的10% FBS之前，不视为完全培养基。血清会提供额外的养分，同时也能够保护悬浮培养条件（即旋转器和振荡培养瓶）下的细胞免受流体压力的影响。

我们推荐您使用我们的Grace’s supplemented昆虫培养基（货号11667037）或SF-900培养基（1.3X）（货号10967032）。
Grace’s Supplemented昆虫培养基是2X的配方培养基，专为在噬斑测定实验琼脂糖覆盖层中的应用而配制。本培养基经以下成份的微调：
乳清蛋白水解物
酵母粉
L-谷氨酰胺
碳酸氢钠
点击此处(https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html)获取完整配方。

SF-900培养基（1.3X）是为Sf9和Sf21细胞准备的不含血清的低蛋白昆虫细胞培养基，适用于在噬斑测定实验1%的琼脂糖覆盖层中使用。

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We would recommend using our Grace's Supplemented Insect Medium (Cat. No. 11667037) or SF-900 Medium (1.3X) (Cat. No. 10967032). Grace's Supplmented Insect Media is a 2X formulation intended for use in the agarose overlay when performing the plaque assay. The media has been modified with:
- Lactalbumin hydrolysate
- Yeastolate
- L-glutamine
- Sodium bicarbonate

Please go here for the complete formulation (http://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html). SF-900 Medium (1.3X) is a complete serum-free low protein insect cell culture medium for Sf9 and Sf21 cells, which can be used for 1% agarose overlays used for plaque assays.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.